Kallikrein 6 ELISA Kit

Produktnummer ABIN2703283

Produktdetails Kallikrein 6 ELISA Kit

Target: Kallikrein 6 (KLK6)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.6-150 ng/mL

Untere Nachweisgrenze: 0.6 ng/mL

Applikation: ELISA

Verwendungszweck: Human Kallikrein 6 ELISA Kit for cell culture supernatants, plasma, and serum samples.

Proben: Plasma, Cell Culture Supernatant, Serum

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair detects human KLK6. Other species not determined.

Sensitivität: 0.6 ng/mL

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Recommended Dilution for serum and plasma samples2 fold

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Referenzen für Kallikrein 6 ELISA Kit (ABIN2703283)

Ehrenfeld, Manso, Pavicic, Matus, Borquez, Lizama, Sarmiento, Poblete, Bhoola, Naran, Figueroa: "Bioregulation of kallikrein-related peptidases 6, 10 and 11 by the kinin B₁ receptor in breast cancer cells." in: Anticancer research, Vol. 34, Issue 12, pp. 6925-38, (2015) (PubMed).

Details zu Kallikrein 6

Target: Kallikrein 6 (KLK6)

Andere Bezeichnung: KLK6 (KLK6 Produkte)

Synonyme: Bssp ELISA Kit, Klk7 ELISA Kit, PRSS18 ELISA Kit, PRSS9 ELISA Kit, SP59 ELISA Kit, hK6 ELISA Kit, Prss9 ELISA Kit, AI849898 ELISA Kit, BSP ELISA Kit, Klk29 ELISA Kit, MSP ELISA Kit, Prss18 ELISA Kit, neurosin ELISA Kit, kallikrein related peptidase 6 ELISA Kit, kallikrein related-peptidase 6 ELISA Kit, kallikrein-6 ELISA Kit, KLK6 ELISA Kit, Klk6 ELISA Kit, LOC100716801 ELISA Kit

Hintergrund: Gene Names: KLK6 PRSS18 PRSS9
Protein names: Kallikrein-6 (EC 3.4.21.-) (Neurosin) (Protease M) (SP59) (Serine protease 18) (Serine protease 9) (Zyme)

Gen-ID: 5653

UniProt: Q92876

Pathways: Komplementsystem, Regulation of G-Protein Coupled Receptor Protein Signaling