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LDLR ELISA Kit
LDLR
Reaktivität: Human
Colorimetric
Sandwich ELISA
8-2.000 pg/mL
Cell Culture Supernatant, Plasma, Serum
Produktnummer ABIN2703289
Produktdetails LDLR ELISA Kit
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Target
Alle LDLR ELISA Kits anzeigen
LDLR
(Low Density Lipoprotein Receptor (LDLR))
Reaktivität
Alle Reaktivitäten für LDLR ELISA Kits
Human
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Detektionsbereich
8-2.000 pg/mL
Untere Nachweisgrenze
8 pg/mL
Applikation
ELISA
Verwendungszweck
Human LDL R ELISA Kit for cell culture supernatants, plasma, and serum samples.
Proben
Cell Culture Supernatant, Plasma, Serum
Analytische Methode
Quantitative
Spezifität
This ELISA antibody pair detects human LDLR. Other species not determined yet.
Sensitivität
8 pg/mL
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Anwendungsinformationen
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Applikationshinweise
Recommended Dilution for serum and plasma samples40-400 fold
Probenmenge
100 μL
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
-20 °C
Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu LDLR
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Target
Alle LDLR ELISA Kits anzeigen
LDLR
(Low Density Lipoprotein Receptor (LDLR))
Andere Bezeichnung
LDL R (LDLR Produkte )
Synonyme
FH ELISA Kit, FHC ELISA Kit, LDLCQ2 ELISA Kit, Hlb301 ELISA Kit, LDLRA ELISA Kit, LDLA ELISA Kit, LDL receptor-2 ELISA Kit, fhc ELISA Kit, ldlcq2 ELISA Kit, ldlr ELISA Kit, ldlr2-a ELISA Kit, LDL receptor 1 ELISA Kit, ldlr-a ELISA Kit, ldlr-b ELISA Kit, low density lipoprotein receptor ELISA Kit, low density lipoprotein receptor a ELISA Kit, low density lipoprotein receptor S homeolog ELISA Kit, low density lipoprotein receptor L homeolog ELISA Kit, LDLR ELISA Kit, Ldlr ELISA Kit, ldlra ELISA Kit, ldlr.S ELISA Kit, ldlr.L ELISA Kit
Hintergrund
Gene Names: LDLR Protein names: Low-density lipoprotein receptor (LDL receptor)
Gen-ID
3949
UniProt
P01130
Pathways
Hepatitis C , Lipid Metabolism
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