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NOV ELISA Kit

NOV Reaktivität: Human Colorimetric Sandwich ELISA 2 pg/mL-30 ng/mL Cell Culture Supernatant, Plasma, Serum
Produktnummer ABIN2703371
  • Target Alle NOV ELISA Kits anzeigen
    NOV (Nephroblastoma Overexpressed (NOV))
    Reaktivität
    • 6
    • 4
    • 2
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    2 pg/mL-30 ng/mL
    Untere Nachweisgrenze
    2 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    Human NOV (CCN3) ELISA Kit for cell culture supernatants, plasma, and serum samples.
    Proben
    Plasma, Cell Culture Supernatant, Serum
    Analytische Methode
    Quantitative
    Spezifität
    This ELISA antibody pair detects human NOV. Other species not determined yet.
    Sensitivität
    2 pg/mL
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Applikationshinweise
    Recommended Dilution for serum and plasma samples2 - 20 fold
    Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Haltbarkeit
    6 months
  • Target Alle NOV ELISA Kits anzeigen
    NOV (Nephroblastoma Overexpressed (NOV))
    Andere Bezeichnung
    NOV (NOV Produkte)
    Synonyme
    CCN3 ELISA Kit, IBP-9 ELISA Kit, IGFBP-9 ELISA Kit, IGFBP9 ELISA Kit, NOVh ELISA Kit, nov-A ELISA Kit, xnov ELISA Kit, NovH ELISA Kit, C130088N23Rik ELISA Kit, nephroblastoma overexpressed ELISA Kit, nephroblastoma overexpressed L homeolog ELISA Kit, nephroblastoma overexpressed gene ELISA Kit, NOV ELISA Kit, nov.L ELISA Kit, Nov ELISA Kit
    Hintergrund
    Gene Names: NOV CCN3 IGFBP9 NOVH
    Protein names: Protein NOV homolog (NovH) (CCN family member 3) (Insulin-like growth factor-binding protein 9) (IBP-9) (IGF-binding protein 9) (IGFBP-9) (Nephroblastoma-overexpressed gene protein homolog)
    Gen-ID
    4856
    UniProt
    P48745
    Pathways
    Smooth Muscle Cell Migration, Growth Factor Binding
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