Osteoactivin ELISA Kit
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- Target Alle Osteoactivin (GPNMB) ELISA Kits anzeigen
- Osteoactivin (GPNMB)
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Reaktivität
- Human
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Detektionsbereich
- 45-12.000 pg/mL
- Untere Nachweisgrenze
- 45 pg/mL
- Applikation
- ELISA
- Verwendungszweck
- Human Osteoactivin (GPNMB) ELISA Kit for cell culture supernatants, plasma, and serum samples.
- Proben
- Plasma, Cell Culture Supernatant, Serum
- Analytische Methode
- Quantitative
- Spezifität
- This antibody pair detects human Osteoactivin. It show less than 20 % cross-reactivity with recombinant mouse Osteoactivin.
- Sensitivität
- 45 pg/mL
- Produktmerkmale
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Bestandteile
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Benötigtes Material
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Applikationshinweise
- Recommended Dilution for serum and plasma samples10 - 110 fold
- Probenmenge
- 100 μL
- Plattentyp
- Pre-coated
- Protokoll
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Lagerung
- -20 °C
- Informationen zur Lagerung
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Haltbarkeit
- 6 months
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- Target Alle Osteoactivin (GPNMB) ELISA Kits anzeigen
- Osteoactivin (GPNMB)
- Andere Bezeichnung
- Osteoactivin (GPNMB Produkte)
- Synonyme
- HGFIN ELISA Kit, NMB ELISA Kit, DC-HIL ELISA Kit, Dchil ELISA Kit, ipd ELISA Kit, glycoprotein nmb ELISA Kit, glycoprotein (transmembrane) nmb ELISA Kit, glycoprotein nmb L homeolog ELISA Kit, Gpnmb ELISA Kit, GPNMB ELISA Kit, gpnmb.L ELISA Kit
- Hintergrund
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Gene Names: GPNMB HGFIN NMB UNQ1725/PRO9925
Protein names: Transmembrane glycoprotein NMB (Transmembrane glycoprotein HGFIN)
- Gen-ID
- 10457
- UniProt
- Q14956
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