Osteocalcin ELISA Kit
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- Target Alle Osteocalcin (BGLAP) ELISA Kits anzeigen
- Osteocalcin (BGLAP)
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Reaktivität
- Human
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Detektionsbereich
- 0.4-100 ng/mL
- Untere Nachweisgrenze
- 0.4 ng/mL
- Applikation
- ELISA
- Verwendungszweck
- Human Osteocalcin ELISA Kit for cell culture supernatants, plasma, and serum samples.
- Proben
- Cell Culture Supernatant, Plasma, Serum
- Analytische Methode
- Quantitative
- Spezifität
- This ELISA antibody pair detects human Osteocalcin. Other species not determined.
- Sensitivität
- 0.4 ng/mL
- Produktmerkmale
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Bestandteile
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Benötigtes Material
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Applikationshinweise
- Recommended Dilution for serum and plasma samples5 fold
- Probenmenge
- 100 μL
- Plattentyp
- Pre-coated
- Protokoll
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Lagerung
- -20 °C
- Informationen zur Lagerung
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Haltbarkeit
- 6 months
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- Target Alle Osteocalcin (BGLAP) ELISA Kits anzeigen
- Osteocalcin (BGLAP)
- Andere Bezeichnung
- Osteocalcin (BGLAP Produkte)
- Synonyme
- BGP ELISA Kit, OC ELISA Kit, OCN ELISA Kit, Bglap1 ELISA Kit, Bglap2 ELISA Kit, OG1 ELISA Kit, mOC-A ELISA Kit, Bgp ELISA Kit, Bgpr ELISA Kit, Bgpra ELISA Kit, bgp ELISA Kit, LOC792433 ELISA Kit, ocn ELISA Kit, GLA ELISA Kit, BGLAP ELISA Kit, PMF1 ELISA Kit, bone gamma-carboxyglutamate protein ELISA Kit, bone gamma carboxyglutamate protein ELISA Kit, bone gamma-carboxyglutamate protein S homeolog ELISA Kit, bone gamma-carboxyglutamate (gla) protein ELISA Kit, osteocalcin ELISA Kit, polyamine-modulated factor 1 ELISA Kit, BGLAP ELISA Kit, Bglap ELISA Kit, bglap.S ELISA Kit, bglap ELISA Kit, ocn ELISA Kit, LOC100529177 ELISA Kit, LOC100146589 ELISA Kit
- Hintergrund
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Gene Names: BGLAP
Protein names: Osteocalcin (Bone Gla protein) (BGP) (Gamma-carboxyglutamic acid-containing protein)
- Gen-ID
- 632
- UniProt
- P02818
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