Periostin ELISA Kit

Produktnummer ABIN2703411

Produktdetails Periostin ELISA Kit

Target: Periostin (POSTN)

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.05-16 ng/mL

Untere Nachweisgrenze: 0.05 ng/mL

Applikation: ELISA

Verwendungszweck: Mouse Periostin ELISA Kit for cell culture supernatants, Heparin and/or EDTA treated plasma, and serum samples. Citrate is not recommended.

Proben: Cell Culture Supernatant, Plasma, Serum

Analytische Methode: Quantitative

Spezifität: This ELISA kit shows no cross-reactivity with the following cytokines tested: Axl, BLC, CD27 Ligand, CD30, CD30 Ligand, CD40, CXCL16, EGF, E-selectin, Fractalkine, GITR, HGF, ICAM-1, IFN-gamma, IGF-1, IGFBP-2, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 ra, IL-1 beta, IL-2, IL-2 R alpha, IL-3, IL-4, IL-5, IL-6, IL-7, IL-10, IL-12 p70, IL-17E, IL-17F, IL-20, IL-23, IL-28, I-TAC, MCP-1, MCP-5, M-CSF, MDC, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 alpha, Osteopontin, Osteoprotegerin, Prolactin, Pro-MMP-9, P-selectin, Resistin, SCF, SDF-1 alpha, TPO, VCAM-1.

Sensitivität: 0.05 ng/mL

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Recommended Dilution for serum and plasma samples50 - 500 fold

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Aufbereitung der Proben:

Collect plasma using heparin and/or EDTA as the anticoagulant. Citrate is not recommended.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu Periostin

Target: Periostin (POSTN)

Andere Bezeichnung: Periostin (POSTN Produkte)

Synonyme: fa99h07 ELISA Kit, pn ELISA Kit, postn ELISA Kit, wu:fa99h07 ELISA Kit, wu:fc70f09 ELISA Kit, zgc:153873 ELISA Kit, LOC100008945 ELISA Kit, osf-2 ELISA Kit, pdlpostn ELISA Kit, periostin ELISA Kit, OSF-2 ELISA Kit, OSF2 ELISA Kit, PDLPOSTN ELISA Kit, PN ELISA Kit, RP11-412K4.1 ELISA Kit, A630052E07Rik ELISA Kit, AI747096 ELISA Kit, Osf2 ELISA Kit, PLF ELISA Kit, peri ELISA Kit, Plf ELISA Kit, periostin ELISA Kit, periostin, osteoblast specific factor b ELISA Kit, periostin, osteoblast specific factor ELISA Kit, POSTN ELISA Kit, postnb ELISA Kit, postn ELISA Kit, Postn ELISA Kit

Hintergrund: Gene Names: Postn Osf2
Protein names: Periostin (PN) (Osteoblast-specific factor 2) (OSF-2)

Gen-ID: 50706

UniProt: Q62009