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TACI ELISA Kit
TNFRSF13B
Reaktivität: Human
Colorimetric
Sandwich ELISA
0.3-80 ng/mL
Cell Culture Supernatant, Plasma, Serum
Produktnummer ABIN2703473
Produktdetails TACI ELISA Kit
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Target
Alle TACI (TNFRSF13B) ELISA Kits anzeigen
TACI (TNFRSF13B)
(Tumor Necrosis Factor Receptor Superfamily, Member 13B (TNFRSF13B))
Reaktivität
Alle Reaktivitäten für TACI ELISA Kits
Human
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Detektionsbereich
0.3-80 ng/mL
Untere Nachweisgrenze
0.3 ng/mL
Applikation
ELISA
Verwendungszweck
Human TACI (TNFRSF13B) ELISA Kit for cell culture supernatants, plasma, and serum samples.
Proben
Cell Culture Supernatant, Plasma, Serum
Analytische Methode
Quantitative
Spezifität
This ELISA antibody pair detects human TACI. Other species not determined.
Sensitivität
0.3 ng/mL
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Anwendungsinformationen
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Applikationshinweise
Recommended Dilution for serum and plasma samples2 fold
Probenmenge
100 μL
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
-20 °C
Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu TACI
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Target
Alle TACI (TNFRSF13B) ELISA Kits anzeigen
TACI (TNFRSF13B)
(Tumor Necrosis Factor Receptor Superfamily, Member 13B (TNFRSF13B))
Andere Bezeichnung
TACI (TNFRSF13B Produkte )
Synonyme
CD267 ELISA Kit, CVID ELISA Kit, CVID2 ELISA Kit, RYZN ELISA Kit, TACI ELISA Kit, TNFRSF14B ELISA Kit, TNFRSF13B ELISA Kit, 1200009E08Rik ELISA Kit, Taci ELISA Kit, TNF receptor superfamily member 13B ELISA Kit, tumor necrosis factor receptor superfamily, member 13b ELISA Kit, TNFRSF13B ELISA Kit, Tnfrsf13b ELISA Kit
Hintergrund
Gene Names: TNFRSF13B TACI Protein names: Tumor necrosis factor receptor superfamily member 13B (Transmembrane activator and CAML interactor) (CD antigen CD267)
Gen-ID
23495
UniProt
O14836
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