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TGFBR2 ELISA Kit
TGFBR2
Reaktivität: Human
Colorimetric
Sandwich ELISA
1.5-500 pg/mL
Cell Culture Supernatant, Plasma, Serum
Produktnummer ABIN2703488
Produktdetails TGFBR2 ELISA Kit
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Target
Alle TGFBR2 ELISA Kits anzeigen
TGFBR2
(Transforming Growth Factor, beta Receptor II (70/80kDa) (TGFBR2))
Reaktivität
Alle Reaktivitäten für TGFBR2 ELISA Kits
Human
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Detektionsbereich
1.5-500 pg/mL
Untere Nachweisgrenze
1.5 pg/mL
Applikation
ELISA
Verwendungszweck
Human TGF beta R2 ELISA Kit for cell culture supernatants, plasma, and serum samples.
Proben
Plasma, Cell Culture Supernatant, Serum
Analytische Methode
Quantitative
Spezifität
This ELISA antibody pair detects human TGF-beta R2. Other species not determined yet.
Sensitivität
15 pg/mL
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Anwendungsinformationen
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Applikationshinweise
Recommended Dilution for serum and plasma samples20 - 200 fold
Probenmenge
100 μL
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
-20 °C
Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu TGFBR2
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Target
Alle TGFBR2 ELISA Kits anzeigen
TGFBR2
(Transforming Growth Factor, beta Receptor II (70/80kDa) (TGFBR2))
Andere Bezeichnung
TGF beta R2 (TGFBR2 Produkte )
Synonyme
AAT3 ELISA Kit, FAA3 ELISA Kit, LDS1B ELISA Kit, LDS2B ELISA Kit, MFS2 ELISA Kit, RIIC ELISA Kit, TAAD2 ELISA Kit, TGFR-2 ELISA Kit, TGFbeta-RII ELISA Kit, 1110020H15Rik ELISA Kit, AU042018 ELISA Kit, DNIIR ELISA Kit, RIIDN ELISA Kit, TBR-II ELISA Kit, TbetaR-II ELISA Kit, TbetaRII ELISA Kit, TGFBR2 ELISA Kit, TBETA-RII ELISA Kit, TGFBRII ELISA Kit, TGF-beta 2 ELISA Kit, Tgfbr2T ELISA Kit, cb537 ELISA Kit, tgfbr2 ELISA Kit, wu:fj05c10 ELISA Kit, zgc:110498 ELISA Kit, transforming growth factor beta receptor 2 ELISA Kit, transforming growth factor, beta receptor II ELISA Kit, transforming growth factor, beta receptor 2 ELISA Kit, transforming growth factor beta receptor 2b ELISA Kit, TGFBR2 ELISA Kit, Tgfbr2 ELISA Kit, tgfbr2b ELISA Kit
Hintergrund
Gene Names: TGFBR2 Protein names: TGF-beta receptor type-2 (TGFR-2) (EC 2.7.11.30) (TGF-beta type II receptor) (Transforming growth factor-beta receptor type II) (TGF-beta receptor type II) (TbetaR-II)
Gen-ID
7048
UniProt
P37173
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