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VEGFR2/CD309 ELISA Kit

VEGFR2 Reaktivität: Maus Colorimetric Sandwich ELISA 80-20.000 pg/mL Cell Culture Supernatant, Plasma, Serum
Produktnummer ABIN2703570
  • Target Alle VEGFR2/CD309 (VEGFR2) ELISA Kits anzeigen
    VEGFR2/CD309 (VEGFR2) (VEGF Receptor 2 (VEGFR2))
    Reaktivität
    • 16
    • 11
    • 9
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Maus
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    80-20.000 pg/mL
    Untere Nachweisgrenze
    80 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    Mouse VEGFR2 ELISA Kit for cell culture supernatants, plasma, and serum samples.
    Proben
    Cell Culture Supernatant, Plasma, Serum
    Analytische Methode
    Quantitative
    Spezifität
    Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30L, CD30, CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN-gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 R beta, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN (OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, TARC, TCA-3, TECK, TIMP-1, TNF RI, TNF RII, TPO, VCAM-1.
    Sensitivität
    80 pg/mL
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Applikationshinweise
    Recommended Dilution for serum and plasma samples2 fold
    Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Haltbarkeit
    6 months
  • Target Alle VEGFR2/CD309 (VEGFR2) ELISA Kits anzeigen
    VEGFR2/CD309 (VEGFR2) (VEGF Receptor 2 (VEGFR2))
    Andere Bezeichnung
    VEGFR2 (VEGFR2 Produkte)
    Synonyme
    CD309 ELISA Kit, FLK1 ELISA Kit, VEGFR ELISA Kit, VEGFR2 ELISA Kit, 6130401C07 ELISA Kit, Flk-1 ELISA Kit, Flk1 ELISA Kit, Krd-1 ELISA Kit, Ly73 ELISA Kit, VEGFR-2 ELISA Kit, sVEGFR-2 ELISA Kit, Vegfr-2 ELISA Kit, FLK-1 ELISA Kit, flk-1 ELISA Kit, vegfr-2 ELISA Kit, KDR ELISA Kit, FLK/KDR ELISA Kit, flk1 ELISA Kit, flk1b ELISA Kit, kdrb ELISA Kit, si:busm1-205d10.1 ELISA Kit, si:ch211-254j6.1 ELISA Kit, si:ch211-278f21.4 ELISA Kit, wu:fc31a09 ELISA Kit, fk52c05 ELISA Kit, flk ELISA Kit, kdr ELISA Kit, kdra ELISA Kit, vegfr2 ELISA Kit, vegfr4 ELISA Kit, vegr2 ELISA Kit, wu:fk52c05 ELISA Kit, kinase insert domain receptor ELISA Kit, kinase insert domain protein receptor ELISA Kit, vascular endothelial growth factor receptor kdr-like ELISA Kit, kinase insert domain receptor (a type III receptor tyrosine kinase) ELISA Kit, kinase insert domain receptor like ELISA Kit, KDR ELISA Kit, Kdr ELISA Kit, LOC100136647 ELISA Kit, kdr ELISA Kit, kdrl ELISA Kit
    Hintergrund
    Gene Names: Kdr
    Protein names: Putative uncharacterized protein (Fragment)
    UniProt
    Q3UQZ6
    Pathways
    RTK Signalweg, Glycosaminoglycan Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, Growth Factor Binding, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling
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