WNK1 ELISA Kit

Produktnummer ABIN2747914

Produktdetails WNK1 ELISA Kit

Target: WNK1 (WNK Lysine Deficient Protein Kinase 1 (WNK1))

Bindungsspezifität: pThr60, total

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human Phospho-WNK1 (T60) & Total WNK1 ELISA Kit. This assay semi-quantitatively measures phophorylated WNK1 (Thr60) & Total WNK1 in lysate samples.

Proben: Cell Lysate, Tissue Lysate

Analytische Methode: Semi-Quantitative

Spezifität: The antibody pair provided in this kit recognizes human WNK1 phosphorylated at site Threonine-60 and total WNK1.

Produktmerkmale:

• Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
• Screen numerous different cell lysates without performing a Western Blot analysis
• Minimal hands-on time, convenient, and non-radioactive material

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-Phospho Antibody
• Anti-Pan Antibody
• HRP-Conjugated Secondary Antibody
• Streptavidin-Conjugated HRP
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

Benötigtes Material:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Anwendungsinformationen

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Testdurchführung:

Prepare all reagents and samples as instructed in the manual.
Add 100 μL of sample or positive control to each well.
Incubate 2.5 h at RT or O/N at 4 °C.
Add 100 μL of prepared primary antibody to each well.
Incubate 1 h at RT.
Add 100 μL of prepared 1X HRP-Streptavidin to each well.
Incubate 1 h at RT.
Add 100 μL of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 μL of Stop Solution to each well.
Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Haltbarkeit: 6 months

Details zu WNK1

Target: WNK1 (WNK Lysine Deficient Protein Kinase 1 (WNK1))

Andere Bezeichnung: WNK1 (WNK1 Produkte)

Synonyme: HSAN2 ELISA Kit, HSN2 ELISA Kit, KDP ELISA Kit, PRKWNK1 ELISA Kit, PSK ELISA Kit, p65 ELISA Kit, WNK1 ELISA Kit, kdp ELISA Kit, psk ELISA Kit, hsn2 ELISA Kit, hsan2 ELISA Kit, pha2c ELISA Kit, prkwnk1 ELISA Kit, 6430573H23Rik ELISA Kit, EG406236 ELISA Kit, Hsn2 ELISA Kit, Prkwnk1 ELISA Kit, mKIAA0344 ELISA Kit, ATWNK1 ELISA Kit, T9J14.14 ELISA Kit, T9J14_14 ELISA Kit, ZIK4 ELISA Kit, with no lysine (K) kinase 1 ELISA Kit, WNK lysine deficient protein kinase 1 ELISA Kit, WNK lysine deficient protein kinase 1 L homeolog ELISA Kit, serine/threonine-protein kinase WNK1 ELISA Kit, with no lysine (K) kinase 1 ELISA Kit, WNK1 ELISA Kit, wnk1.L ELISA Kit, wnk1 ELISA Kit, LOC100056745 ELISA Kit, Wnk1 ELISA Kit

Hintergrund: WNK Lysine-deficient Protein Kinase 1 (WNK1, KDP, PHA2C, PRKWNK1) phosphorylated at Threonine-60 & total WNK1

Gen-ID: 65125

UniProt: Q9H4A3