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DLL1 ELISA Kit

DLL1 Reaktivität: Ratte Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Produktnummer ABIN2748051
  • Target Alle DLL1 ELISA Kits anzeigen
    DLL1 (delta-Like 1 (DLL1))
    Reaktivität
    • 7
    • 3
    • 2
    Ratte
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Custom Rat DLL1 ELISA Kit.
    Proben
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytische Methode
    Quantitative
    Spezifität
    This ELISA antibody pair recognizes Rat DLL1. Other species not yet determined.
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Haltbarkeit
    6 months
  • Target Alle DLL1 ELISA Kits anzeigen
    DLL1 (delta-Like 1 (DLL1))
    Andere Bezeichnung
    DLL1 (DLL1 Produkte)
    Synonyme
    X-delta-1 ELISA Kit, XDelta1 ELISA Kit, Xdelta-1 ELISA Kit, delta ELISA Kit, delta-1 ELISA Kit, delta1 ELISA Kit, x-delta ELISA Kit, DELTA1 ELISA Kit, DL1 ELISA Kit, Delta ELISA Kit, DLK ELISA Kit, DLK-1 ELISA Kit, Delta1 ELISA Kit, FA1 ELISA Kit, PREF1 ELISA Kit, Pref-1 ELISA Kit, ZOG ELISA Kit, pG2 ELISA Kit, DLL1 ELISA Kit, AW742678 ELISA Kit, DlkI ELISA Kit, Ly107 ELISA Kit, Peg9 ELISA Kit, SCP1 ELISA Kit, pref-1 ELISA Kit, delta-like 1 ELISA Kit, delta like canonical Notch ligand 1 ELISA Kit, delta like non-canonical Notch ligand 1 ELISA Kit, delta-like 1 L homeolog ELISA Kit, delta-like 1 (Drosophila) ELISA Kit, delta-like 1 homolog (Drosophila) ELISA Kit, dll1 ELISA Kit, DLL1 ELISA Kit, DLK1 ELISA Kit, Dll1 ELISA Kit, dll1.L ELISA Kit, Dlk1 ELISA Kit
    Hintergrund
    Delta-like protein 1 (Drosophila Delta homolog 1) (Delta1)
    Gen-ID
    84010
    UniProt
    P97677
    Pathways
    Notch Signalweg, Stem Cell Maintenance
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