ERK2 ELISA Kit

Produktnummer ABIN2748087

Produktdetails ERK2 ELISA Kit

Target: ERK2 (MAPK1) (Mitogen-Activated Protein Kinase 1 (MAPK1))

Bindungsspezifität: pThr185, pTyr187, total

Reaktivität: Human, Ratte, Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human/Mouse/Rat Phospho-Erk2 (T185/Y187) and Total ERK2 ELISA Kit. This assay semi-quantitatively measures phophorylated Erk2 (Thr185/Tyr187) and Total ERK2 in lysate samples.

Proben: Cell Lysate, Tissue Lysate

Analytische Methode: Semi-Quantitative

Spezifität: The antibody pair provided in this kit recognizes human, mouse, or rat Phospho-ERK2 (pThr185/pTyr187) and total ERK2.

Produktmerkmale:

• Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
• Screen numerous different cell lysates without performing a Western Blot analysis
• Minimal hands-on time, convenient, and non-radioactive material

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-Phospho Antibody
• Anti-Pan Antibody
• HRP-Conjugated Secondary Antibody
• Streptavidin-Conjugated HRP
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

Benötigtes Material:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Anwendungsinformationen

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Testdurchführung:

Prepare all reagents and samples as instructed in the manual.
Add 100 μL of sample or positive control to each well.
Incubate 2.5 h at RT or O/N at 4 °C.
Add 100 μL of prepared primary antibody to each well.
Incubate 1 h at RT.
Add 100 μL of prepared 1X HRP-Streptavidin to each well.
Incubate 1 h at RT.
Add 100 μL of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 μL of Stop Solution to each well.
Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Haltbarkeit: 6 months

Details zu ERK2

Target: ERK2 (MAPK1) (Mitogen-Activated Protein Kinase 1 (MAPK1))

Andere Bezeichnung: Erk2 (MAPK1 Produkte)

Synonyme: ERK-1 ELISA Kit, ERK1 ELISA Kit, ERT2 ELISA Kit, HS44KDAP ELISA Kit, HUMKER1A ELISA Kit, P44ERK1 ELISA Kit, P44MAPK ELISA Kit, PRKM3 ELISA Kit, p44-ERK1 ELISA Kit, p44-MAPK ELISA Kit, Erk-1 ELISA Kit, Erk1 ELISA Kit, Ert2 ELISA Kit, Esrk1 ELISA Kit, Mnk1 ELISA Kit, Mtap2k ELISA Kit, Prkm3 ELISA Kit, p44 ELISA Kit, p44erk1 ELISA Kit, p44mapk ELISA Kit, ERK ELISA Kit, ERK2 ELISA Kit, ERT1 ELISA Kit, MAPK2 ELISA Kit, P42MAPK ELISA Kit, PRKM1 ELISA Kit, PRKM2 ELISA Kit, p38 ELISA Kit, p40 ELISA Kit, p41 ELISA Kit, p41mapk ELISA Kit, 9030612K14Rik ELISA Kit, AA407128 ELISA Kit, AU018647 ELISA Kit, C78273 ELISA Kit, Erk2 ELISA Kit, Prkm1 ELISA Kit, p42mapk ELISA Kit, zERK2 ELISA Kit, MAPK1 ELISA Kit, Xp42 ELISA Kit, erk ELISA Kit, erk2 ELISA Kit, ert1 ELISA Kit, mapk ELISA Kit, mapk1b ELISA Kit, mapk2 ELISA Kit, mpk1 ELISA Kit, prkm1 ELISA Kit, prkm2 ELISA Kit, mapk1 ELISA Kit, mapk1a ELISA Kit, xp42 ELISA Kit, ATMPK1 ELISA Kit, F14N23.9 ELISA Kit, F14N23_9 ELISA Kit, MITOGEN-ACTIVATED PROTEIN KINASE ELISA Kit, mitogen-activated protein kinase 1 ELISA Kit, MNK1 ELISA Kit, ATMPK2 ELISA Kit, MITOGEN-ACTIVATED PROTEIN KINASE HOMOLOG 2 ELISA Kit, T30E16.13 ELISA Kit, T30E16_13 ELISA Kit, mitogen-activated protein kinase homolog 2 ELISA Kit, mitogen-activated protein kinase 3 ELISA Kit, mitogen-activated protein kinase 1 ELISA Kit, mitogen activated protein kinase 1 ELISA Kit, mitogen-activated protein kinase 1 L homeolog ELISA Kit, mitogen-activated protein kinase 1 S homeolog ELISA Kit, mitogen activated protein kinase 3 ELISA Kit, mitogen-activated protein kinase homolog 2 ELISA Kit, MAPK3 ELISA Kit, Mapk3 ELISA Kit, MAPK1 ELISA Kit, Mapk1 ELISA Kit, mapk1 ELISA Kit, mapk1.L ELISA Kit, mapk1.S ELISA Kit, PfMAP1 ELISA Kit, MPK1 ELISA Kit, PVX_084965 ELISA Kit, LOC9328617 ELISA Kit, Tsp_01601 ELISA Kit, MPK2 ELISA Kit

Hintergrund: ERK2, Mitogen-activated protein kinase 1, MAPK1, p42MAPK

Gen-ID: 5594

UniProt: P28482

Pathways: MAPK Signalweg, RTK Signalweg, Apoptose, Interferon-gamma Pathway, Fc-epsilon Rezeptor Signalübertragung, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Monocarboxylic Acid Catabolic Process, Autophagie, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, BCR Signaling, S100 Proteine