HSP27 ELISA Kit

Produktnummer ABIN2748167

Produktdetails HSP27 ELISA Kit

Target: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Bindungsspezifität: pSer82

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human Phopspho-HSP27 (S82) ELISA Kit. This assay semi-quantitatively measures phophorylated HSP27 (Ser82) in lysate samples.

Proben: Cell Lysate, Tissue Lysate

Analytische Methode: Semi-Quantitative

Spezifität: The antibody pair provided in this kit recognizes human Phospho-HSP27 (pSer82).

Produktmerkmale:

• Rapidly measure phosphorylated protein in lysates
• Screen numerous different cell lysates without performing a Western Blot analysis
• Minimal hands-on time, convenient, and non-radioactive material

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-Phospho Antibody
• HRP-Conjugated Secondary Antibody
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

Benötigtes Material:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Anwendungsinformationen

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Testdurchführung:

Prepare all reagents and samples as instructed in the manual.
Add 100 μL of sample or positive control to each well.
Incubate 2.5 h at RT or O/N at 4 °C.
Add 100 μL of prepared primary antibody to each well.
Incubate 1 h at RT.
Add 100 μL of prepared 1X HRP-Streptavidin to each well.
Incubate 1 h at RT.
Add 100 μL of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 μL of Stop Solution to each well.
Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Haltbarkeit: 6 months

Details zu HSP27

Target: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Andere Bezeichnung: HSP27 (HSPB1 Produkte)

Synonyme: CMT2F ELISA Kit, HMN2B ELISA Kit, HS.76067 ELISA Kit, HSP27 ELISA Kit, HSP28 ELISA Kit, Hsp25 ELISA Kit, SRP27 ELISA Kit, 27kDa ELISA Kit, Hsp27 ELISA Kit, cb153 ELISA Kit, cb660 ELISA Kit, hsp1 ELISA Kit, hsp25 ELISA Kit, hsp27 ELISA Kit, id:ibd2821 ELISA Kit, sb:cb660 ELISA Kit, zgc:103437 ELISA Kit, HSPB1 ELISA Kit, cmt2f ELISA Kit, hs.76067 ELISA Kit, hsp28 ELISA Kit, heat shock protein family B (small) member 1 ELISA Kit, heat shock protein 1 ELISA Kit, heat shock protein, alpha-crystallin-related, 1 ELISA Kit, heat shock protein family B (small) member 1 L homeolog ELISA Kit, HSPB1 ELISA Kit, Hspb1 ELISA Kit, hspb1 ELISA Kit, hspb1.L ELISA Kit

Hintergrund: Heat Shock Protein 27 (Hsp27), Heat Shock Protein Beta-1 (HSPB1) phosphorylated at S82

Gen-ID: 3315

UniProt: P04792

Pathways: MAPK Signalweg, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling