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IGFBP3 ELISA Kit

IGFBP3 Reaktivität: Rhesusaffen Colorimetric Sandwich ELISA 1.02 ng/mL Cell Culture Supernatant, Plasma, Serum
Produktnummer ABIN2748197
  • Target Alle IGFBP3 ELISA Kits anzeigen
    IGFBP3 (Insulin-Like Growth Factor Binding Protein 3 (IGFBP3))
    Reaktivität
    • 11
    • 8
    • 4
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Rhesusaffen
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    1.02 ng/mL
    Untere Nachweisgrenze
    1.02 ng/mL
    Applikation
    ELISA
    Verwendungszweck
    Rhesus Monkey IGFBP-3 ELISA Kit for serum, plasma, and cell culture supernatant samples.
    Proben
    Plasma, Cell Culture Supernatant, Serum
    Analytische Methode
    Quantitative
    Spezifität
    This ELISA antibody pair detects Rhesus Macaque IGFBP-3 and human IGFBP-3. Other species not determined.
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Applikationshinweise
    Recommended Dilution for serum and plasma samples2 fold
    Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Testdurchführung

    Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Haltbarkeit
    6 months
  • Target Alle IGFBP3 ELISA Kits anzeigen
    IGFBP3 (Insulin-Like Growth Factor Binding Protein 3 (IGFBP3))
    Andere Bezeichnung
    IGFBP-3 (IGFBP3 Produkte)
    Synonyme
    BP-53 ELISA Kit, IBP3 ELISA Kit, AI649005 ELISA Kit, IGFBP-3 ELISA Kit, IGgfbp3 ELISA Kit, IGF-BP3 ELISA Kit, zgc:91788 ELISA Kit, IGFBP3 ELISA Kit, igfbp3 ELISA Kit, insulin like growth factor binding protein 3 ELISA Kit, insulin-like growth factor binding protein 3 ELISA Kit, IGFBP3 ELISA Kit, Igfbp3 ELISA Kit, igfbp3 ELISA Kit, IGFBP-3 ELISA Kit, LOC100305016 ELISA Kit
    Hintergrund
    Insulin-like Growth Factor Binding Protein 3 (IGFBP3)
    UniProt
    F6U2J6
    Pathways
    Myometrial Relaxation and Contraction, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagie, Smooth Muscle Cell Migration, Growth Factor Binding
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