IGFBP5 ELISA Kit

Produktnummer ABIN2748200

Produktdetails IGFBP5 ELISA Kit

Target: IGFBP5 (Insulin-Like Growth Factor Binding Protein 5 (IGFBP5))

Reaktivität: Schwein

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Custom Porcine (pig) Insulin-like Growth Factor Binding Protein 5 (IGFBP5) ELISA Kit.

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Spezifität: The antibody pair provided in this kit recognizes Porcine Insulin-like Growth Factor Binding Protein 5 (IGFBP5)

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Testdurchführung:

Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu IGFBP5

Target: IGFBP5 (Insulin-Like Growth Factor Binding Protein 5 (IGFBP5))

Andere Bezeichnung: IGFBP-5 (IGFBP5 Produkte)

Synonyme: IBP5 ELISA Kit, AI256729 ELISA Kit, AW208790 ELISA Kit, IGFBP-5 ELISA Kit, IGFBP-5P ELISA Kit, IGF-BP5 ELISA Kit, igfbp5 ELISA Kit, ibp5 ELISA Kit, IBP-5 ELISA Kit, xIGFBP-5 ELISA Kit, IGFBP5 ELISA Kit, igfbp5b ELISA Kit, zgc:165472 ELISA Kit, insulin like growth factor binding protein 5 ELISA Kit, insulin-like growth factor binding protein 5 ELISA Kit, insulin like growth factor binding protein 5 L homeolog ELISA Kit, insulin-like growth factor binding protein 5b ELISA Kit, insulin-like growth factor-binding protein 5 ELISA Kit, insulin-like growth factor binding protein 5a ELISA Kit, IGFBP5 ELISA Kit, Igfbp5 ELISA Kit, igfbp5.L ELISA Kit, igfbp5b ELISA Kit, igfbp5 ELISA Kit, LOC100194500 ELISA Kit, igfbp5a ELISA Kit

Hintergrund: Insulin-like Growth Factor Binding Protein 5 (IGFBP5)

Gen-ID: 397182

UniProt: Q28985

Pathways: WNT Signalweg, Carbohydrate Homeostasis, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process, Autophagie, Smooth Muscle Cell Migration, Growth Factor Binding