JNK ELISA Kit
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- Target Alle JNK (MAPK8) ELISA Kits anzeigen
- JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))
- Bindungsspezifität
- pThr183, pTyr185
- Reaktivität
- Human, Maus, Ratte
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Applikation
- ELISA
- Verwendungszweck
- Human/Mouse/Rat Phospho-JNK (T183/Y185) ELISA Kit. This assay semi-quantitatively measures phophorylated JNK (Thr183/Tyr185) in lysate samples.
- Proben
- Cell Lysate, Tissue Lysate
- Analytische Methode
- Semi-Quantitative
- Spezifität
- The antibody pair provided in this kit recognizes Human/Mouse/Rat Phospho-JNK (pThr183/Tyr185).
- Produktmerkmale
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- Rapidly measure phosphorylated protein in lysates
- Screen numerous different cell lysates without performing a Western Blot analysis
- Minimal hands-on time, convenient, and non-radioactive material
- Bestandteile
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Anti-Phospho Antibody
- HRP-Conjugated Secondary Antibody
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Lysis Buffer
- Positive Control Sample
- Benötigtes Material
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
- Featured
- Zu unserem meistverkauften MAPK8 ELISA Kit
- Top Product
- Discover our top product MAPK8 ELISA Kit
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- Probenmenge
- 100 μL
- Plattentyp
- Pre-coated
- Protokoll
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- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Testdurchführung
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Prepare all reagents and samples as instructed in the manual.
Add 100 μL of sample or positive control to each well.
Incubate 2.5 h at RT or O/N at 4 °C.
Add 100 μL of prepared primary antibody to each well.
Incubate 1 h at RT.
Add 100 μL of prepared 1X HRP-Streptavidin to each well.
Incubate 1 h at RT.
Add 100 μL of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 μL of Stop Solution to each well.
Read at 450 nm immediately. - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Lagerung
- -20 °C
- Informationen zur Lagerung
- Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
- Haltbarkeit
- 6 months
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Inhibitory effects of pirfenidone on dendritic cells and lung allograft rejection." in: Transplantation, Vol. 94, Issue 2, pp. 114-22, (2012) (PubMed).
: "Myocyte enhancer factor 2c, an osteoblast transcription factor identified by dimethyl sulfoxide (DMSO)-enhanced mineralization." in: The Journal of biological chemistry, Vol. 286, Issue 34, pp. 30071-86, (2011) (PubMed).
: "
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Inhibitory effects of pirfenidone on dendritic cells and lung allograft rejection." in: Transplantation, Vol. 94, Issue 2, pp. 114-22, (2012) (PubMed).
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- Target Alle JNK (MAPK8) ELISA Kits anzeigen
- JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))
- Andere Bezeichnung
- JNK (MAPK8 Produkte)
- Synonyme
- JNK ELISA Kit, JNK-46 ELISA Kit, JNK1 ELISA Kit, JNK1A2 ELISA Kit, JNK21B1/2 ELISA Kit, PRKM8 ELISA Kit, SAPK1 ELISA Kit, SAPK1c ELISA Kit, AI849689 ELISA Kit, Prkm8 ELISA Kit, BSK ELISA Kit, BSK/DJNK ELISA Kit, Bsk ELISA Kit, CG5680 ELISA Kit, D-JNK ELISA Kit, D-junk ELISA Kit, DBSK/JNK ELISA Kit, DJNK ELISA Kit, DJNK/bsk ELISA Kit, Dmel\\CG5680 ELISA Kit, JNK/SAPK ELISA Kit, Jnk ELISA Kit, Junk ELISA Kit, SAPKa ELISA Kit, c-Jun ELISA Kit, dJNK ELISA Kit, jnk ELISA Kit, T10F20.15 ELISA Kit, jnk1 ELISA Kit, sapk1 ELISA Kit, mapk8 ELISA Kit, zgc:112379 ELISA Kit, JNKb ELISA Kit, mapk8b ELISA Kit, mitogen-activated protein kinase 8 ELISA Kit, Stress-activated protein kinase jnk-1 ELISA Kit, basket ELISA Kit, Protein kinase superfamily protein ELISA Kit, mitogen-activated protein kinase 8 L homeolog ELISA Kit, mitogen-activated protein kinase 8b ELISA Kit, mitogen-activated protein kinase 8B-like ELISA Kit, MAPK8 ELISA Kit, jnk-1 ELISA Kit, Mapk8 ELISA Kit, bsk ELISA Kit, ATMPK8 ELISA Kit, mapk8.L ELISA Kit, mapk8b ELISA Kit, LOC109094263 ELISA Kit
- Hintergrund
- C-Jun N-terminal kinase (JNK) phosphorylated at T183 and/or Y185
- Gen-ID
- 5599
- UniProt
- P45983
- Pathways
- MAPK Signalweg, WNT Signalweg, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, S100 Proteine
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