Reticulon 4 ELISA Kit

Produktnummer ABIN2748436

Produktdetails Reticulon 4 ELISA Kit

Target: Reticulon 4 (RTN4)

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Custom Rat Nogo-A (Reticulon 4A) ELISA Kit.

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes Rat Nogo-A. Other species not yet determined.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu Reticulon 4

Target: Reticulon 4 (RTN4)

Andere Bezeichnung: Nogo-A (RTN4 Produkte)

Synonyme: ASY ELISA Kit, NI220/250 ELISA Kit, NOGO ELISA Kit, NOGO-A ELISA Kit, NOGOC ELISA Kit, NSP ELISA Kit, NSP-CL ELISA Kit, Nbla00271 ELISA Kit, Nbla10545 ELISA Kit, Nogo-B ELISA Kit, Nogo-C ELISA Kit, RTN-X ELISA Kit, RTN4-A ELISA Kit, RTN4-B1 ELISA Kit, RTN4-B2 ELISA Kit, RTN4-C ELISA Kit, RTN4-Cw ELISA Kit, DKFZp459C0314 ELISA Kit, 1110020G17Rik ELISA Kit, AA407876 ELISA Kit, AA409940 ELISA Kit, AA960376 ELISA Kit, C130026I10Rik ELISA Kit, NgA ELISA Kit, Nogo-A ELISA Kit, mKIAA0886 ELISA Kit, mKIAA4153 ELISA Kit, NI-250 ELISA Kit, Nogo ELISA Kit, Vp20 ELISA Kit, r ELISA Kit, rat N ELISA Kit, rat NogoA ELISA Kit, reticulon-4 ELISA Kit, nogo ELISA Kit, rtn4 ELISA Kit, wu:fb17a02 ELISA Kit, wu:fb22f12 ELISA Kit, wu:fd61a07 ELISA Kit, zgc:92163 ELISA Kit, reticulon 4 ELISA Kit, reticulon 4a ELISA Kit, RTN4 ELISA Kit, rtn4 ELISA Kit, Rtn4 ELISA Kit, rtn4a ELISA Kit

Hintergrund: Reticulon-4 (Foocen) (Glut4 vesicle 20 kDa protein) (Neurite outgrowth inhibitor) (Nogo protein)

Gen-ID: 83765

UniProt: Q9JK11

Pathways: Neurotrophin Signalübertragung, Regulation of Cell Size, SARS-CoV-2 Protein Interaktom