Notch1 ELISA Kit

Produktnummer ABIN2748442

Produktdetails Notch1 ELISA Kit

Target: Notch1 (NOTCH1) (Notch 1 (NOTCH1))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Custom Rat Notch-1 ELISA Kit.

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Spezifität: The antibody pair provided in this kit recognizes Rat Notch-1

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Testdurchführung:

Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu Notch1

Target: Notch1 (NOTCH1) (Notch 1 (NOTCH1))

Andere Bezeichnung: Notch-1 (NOTCH1 Produkte)

Synonyme: notch1 ELISA Kit, NOTCH1 ELISA Kit, hn1 ELISA Kit, tan1 ELISA Kit, notch ELISA Kit, xotch ELISA Kit, xnotch ELISA Kit, notch-1 ELISA Kit, xnotch1 ELISA Kit, TAN1 ELISA Kit, hN1 ELISA Kit, 9930111A19Rik ELISA Kit, Mis6 ELISA Kit, N1 ELISA Kit, Tan1 ELISA Kit, lin-12 ELISA Kit, NOTCH ELISA Kit, Xotch ELISA Kit, notch1-a ELISA Kit, notch 1 ELISA Kit, fibropellin-1 ELISA Kit, Notch homolog 1, translocation-associated (Drosophila) ELISA Kit, Notch homolog 1, translocation-associated ELISA Kit, notch 1 S homeolog ELISA Kit, notch1 ELISA Kit, LOC100631526 ELISA Kit, NOTCH1 ELISA Kit, Notch1 ELISA Kit, notch1.S ELISA Kit

Hintergrund: Notch-1

Gen-ID: 25496

UniProt: Q07008

Pathways: Notch Signalweg, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development