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Notch1 ELISA Kit

NOTCH1 Reaktivität: Ratte Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Produktnummer ABIN2748442
  • Target Alle Notch1 (NOTCH1) ELISA Kits anzeigen
    Notch1 (NOTCH1) (Notch 1 (NOTCH1))
    Reaktivität
    • 4
    • 2
    • 2
    Ratte
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Custom Rat Notch-1 ELISA Kit.
    Proben
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytische Methode
    Quantitative
    Spezifität
    The antibody pair provided in this kit recognizes Rat Notch-1
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Testdurchführung

    Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Haltbarkeit
    6 months
  • Target Alle Notch1 (NOTCH1) ELISA Kits anzeigen
    Notch1 (NOTCH1) (Notch 1 (NOTCH1))
    Andere Bezeichnung
    Notch-1 (NOTCH1 Produkte)
    Synonyme
    notch1 ELISA Kit, NOTCH1 ELISA Kit, hn1 ELISA Kit, tan1 ELISA Kit, notch ELISA Kit, xotch ELISA Kit, xnotch ELISA Kit, notch-1 ELISA Kit, xnotch1 ELISA Kit, TAN1 ELISA Kit, hN1 ELISA Kit, 9930111A19Rik ELISA Kit, Mis6 ELISA Kit, N1 ELISA Kit, Tan1 ELISA Kit, lin-12 ELISA Kit, NOTCH ELISA Kit, Xotch ELISA Kit, notch1-a ELISA Kit, notch 1 ELISA Kit, fibropellin-1 ELISA Kit, Notch homolog 1, translocation-associated (Drosophila) ELISA Kit, Notch homolog 1, translocation-associated ELISA Kit, notch 1 S homeolog ELISA Kit, notch1 ELISA Kit, LOC100631526 ELISA Kit, NOTCH1 ELISA Kit, Notch1 ELISA Kit, notch1.S ELISA Kit
    Hintergrund
    Notch-1
    Gen-ID
    25496
    UniProt
    Q07008
    Pathways
    Notch Signalweg, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development
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