CDKN1B ELISA Kit

Produktnummer ABIN2748476

Produktdetails CDKN1B ELISA Kit

Target: CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Custom Rat p27/Kip1 (CDKN1B) ELISA Kit.

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Spezifität: The antibody pair provided in this kit recognizes Rat p27/Kip1 (CDKN1B)

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Testdurchführung:

Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu CDKN1B

Target: CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))

Andere Bezeichnung: p27/Kip1 (CDKN1B Produkte)

Synonyme: CDKN4 ELISA Kit, KIP1 ELISA Kit, MEN1B ELISA Kit, MEN4 ELISA Kit, P27KIP1 ELISA Kit, cdkn1b ELISA Kit, sb:cb611 ELISA Kit, wu:fb63g10 ELISA Kit, wu:fb64g10 ELISA Kit, wu:fe18e03 ELISA Kit, CDKN1B ELISA Kit, Kip1 ELISA Kit, p27 ELISA Kit, FAM14D ELISA Kit, ISG12 ELISA Kit, ISG12A ELISA Kit, P27 ELISA Kit, Cdki1b ELISA Kit, AA408329 ELISA Kit, AI843786 ELISA Kit, p27Kip1 ELISA Kit, cdkn1bl ELISA Kit, cyclin dependent kinase inhibitor 1B ELISA Kit, cyclin-dependent kinase inhibitor 1Bb ELISA Kit, interferon alpha inducible protein 27 ELISA Kit, cyclin-dependent kinase inhibitor 1B ELISA Kit, cyclin-dependent kinase inhibitor 1Ba ELISA Kit, CDKN1B ELISA Kit, cdkn1bb ELISA Kit, IFI27 ELISA Kit, Cdkn1b ELISA Kit, cdkn1ba ELISA Kit

Hintergrund: P27/Kip1 (CDKN1B)

Gen-ID: 83571

UniProt: O08769

Pathways: Zellzyklus, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Sensory Perception of Sound, Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Synthesis of DNA, Autophagie