TIMP2 ELISA Kit
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- Target Alle TIMP2 ELISA Kits anzeigen
- TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))
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Reaktivität
- Ratte
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Detektionsbereich
- 20-5.000 pg/mL
- Untere Nachweisgrenze
- 20 pg/mL
- Applikation
- ELISA
- Verwendungszweck
- Rat TIMP-2 ELISA Kit for cell culture supernatants, plasma, and serum samples.
- Proben
- Plasma, Cell Culture Supernatant, Serum
- Analytische Methode
- Quantitative
- Spezifität
- Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: rat 4-1BB, Activin A, Adiponectin, B7-1, CD48, CTACK, Decorin, Eotaxin, EphA5, Erythropoietin, FGF-BP, Fit-3 Ligand, Galectin-1, Galectin-3, Gas 1, GFR alpha-1, gp130, HGF, IL-1 R6, IL-1 ra, IL-2 R alpha, IL-3, IL-7, IL-17F, IL-22, JAM-A, MIP-1 alpha, Neuropilin-1, Neuropilin-2, Nope, Notch-1, Notch-2, P-Cadherin, Prolactin, RANTES, SCF, TIM-1, TREM-1, TWEAK R .
- Sensitivität
- 20 pg/mL
- Produktmerkmale
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Bestandteile
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Benötigtes Material
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Applikationshinweise
- Recommended Dilution for serum and plasma samples2-10 fold
- Probenmenge
- 100 μL
- Plattentyp
- Pre-coated
- Protokoll
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Testdurchführung
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Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Lagerung
- -20 °C
- Informationen zur Lagerung
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Haltbarkeit
- 6 months
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- Target Alle TIMP2 ELISA Kits anzeigen
- TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))
- Andere Bezeichnung
- TIMP-2 (TIMP2 Produkte)
- Synonyme
- CSC-21K ELISA Kit, DDC8 ELISA Kit, timp2l ELISA Kit, zgc:65967 ELISA Kit, zgc:85799 ELISA Kit, D11Bwg1104e ELISA Kit, Timp-2 ELISA Kit, TIMP-2 ELISA Kit, etID32613.12 ELISA Kit, fa96h09 ELISA Kit, timp2 ELISA Kit, wu:fa96h09 ELISA Kit, zgc:136726 ELISA Kit, TIMP metallopeptidase inhibitor 2 ELISA Kit, TIMP metallopeptidase inhibitor 2b ELISA Kit, tissue inhibitor of metalloproteinase 2 ELISA Kit, TIMP metallopeptidase inhibitor 2a ELISA Kit, TIMP metallopeptidase inhibitor 2 L homeolog ELISA Kit, TIMP2 ELISA Kit, timp2b ELISA Kit, Timp2 ELISA Kit, timp2a ELISA Kit, timp2.L ELISA Kit
- Hintergrund
- Metalloproteinase inhibitor 2 (Tissue inhibitor of metalloproteinases 2) (TIMP-2)
- Gen-ID
- 29543
- UniProt
- P30121
- Pathways
- cAMP Metabolic Process
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