Biglycan ELISA Kit

Produktnummer ABIN2859228

Produktdetails Biglycan ELISA Kit

Target: Biglycan (BGN)

Bindungsspezifität: AA 38-368

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 156-10.000 pg/mL

Untere Nachweisgrenze: 156 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Biglycan

Marke: PicoKine™

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NSO
Immunogen sequence: D38-K368

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <10pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: D38-K368

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Sequence similarities: Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.

Tissue Specificity: Found in several connective tissues, especially in articular cartilages.

Plattentyp: Pre-coated

Protokoll: human Biglycan ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Biglycan has been precoated onto 96-well plates. Standards(NSO, D38-K368) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Biglycan is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Biglycan amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Biglycan standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or tissue homogenates to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Biglycan standard solution and each sample be measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(ng/ml): 1.62, Standard deviation: 0.06, CV(%): 3.7
• Sample 2: n=16, Mean(ng/ml): 2.91, Standard deviation: 0.157, CV(%): 5.4
• Sample 3: n=16, Mean(ng/ml): 5.38, Standard deviation: 0.264, CV(%): 4.9,
• Sample 1: n=24, Mean(ng/ml): 1.52, Standard deviation: 0.062, CV(%): 4.1
• Sample 2: n=24, Mean(ng/ml): 3.07, Standard deviation: 0.209, CV(%): 6.8
• Sample 3: n=24, Mean(ng/ml): 6.34, Standard deviation: 0.33, CV(%): 5.2

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Details zu Biglycan

Target: Biglycan (BGN)

Andere Bezeichnung: BGN (BGN Produkte)

Synonyme: DSPG1 ELISA Kit, PG-S1 ELISA Kit, PGI ELISA Kit, SLRR1A ELISA Kit, BG ELISA Kit, BSPG1 ELISA Kit, bgn ELISA Kit, zgc:91989 ELISA Kit, dspg1 ELISA Kit, pg-s1 ELISA Kit, slrr1a ELISA Kit, xbgn ELISA Kit, BGN ELISA Kit, zgc:77123 ELISA Kit, DKFZp468I2427 ELISA Kit, biglycan ELISA Kit, biglycan L homeolog ELISA Kit, biglycan a ELISA Kit, biglycan S homeolog ELISA Kit, biglycan b ELISA Kit, BGN ELISA Kit, Bgn ELISA Kit, bgn.L ELISA Kit, bgna ELISA Kit, bgn.S ELISA Kit, bgn ELISA Kit, bgnb ELISA Kit

Hintergrund:

Protein Function: May be involved in collagen fiber assembly. .

Background: Biglycan (BGN) is a small leucine-rich repeat proteoglycan (SLRP) which is found in a variety of extracellular matrix tissues, including bone, cartilage and tendon. It is an important structural component of articular cartilage and participates in the assembly of the chondrocyte extracellular matrix through formation of protein interactions with type VI collagen and large proteoglycan aggregates. In humans, Biglycan is encoded by the BGN gene. It is mapped to Xq28. Biglycan is believed to play a role in the mineralisation of bone, and its core protein binds to the growth factors BMP-4 and influences its bioactivity. It has been reported that the presence of biglycan is necessary for BMP-4 to exert its effects on osteoblasts. There is also evidence that biglycan can bind to TGF-beta 1. In addition, Biglycan plays an important role in cell signaling.

Synonyms: Biglycan,Bone/cartilage proteoglycan I,PG-S1,BGN,SLRR1A,

Full Gene Name: Biglycan

Cellular Localisation: Secreted, extracellular space, extracellular matrix.

Gen-ID: 633

UniProt: P21810

Pathways: Glycosaminoglycan Metabolic Process