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PLTP ELISA Kit

PLTP Reaktivität: Maus AA 18-493 Colorimetric Sandwich ELISA 0.78-50 ng/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Produktnummer ABIN2859237
  • Target Alle PLTP ELISA Kits anzeigen
    PLTP (Phospholipid Transfer Protein (PLTP))
    Bindungsspezifität
    AA 18-493
    Reaktivität
    • 6
    • 5
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Maus
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.78-50 ng/mL
    Untere Nachweisgrenze
    0.78 ng/mL
    Applikation
    ELISA
    Verwendungszweck
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse PLTP
    Marke
    PicoKine™
    Proben
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytische Methode
    Quantitative
    Spezifität
    Expression system for standard: NSO
    Immunogen sequence: E18-A493
    Kreuzreaktivität (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivität
    <20pg/mL
    Benötigtes Material
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: NSO
    Immunogen sequence: E18-A493
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  • Applikationshinweise
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Kommentare

    Tissue Specificity: Highest in lung, adipose tissue, brain, and heart.

    Plattentyp
    Pre-coated
    Protokoll
    mouse PLTP ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for PLTP has been precoated onto 96-well plates. Standards(NSO, E18-A493) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PLTP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse PLTP amount of sample captured in plate.
    Testdurchführung

    Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL mouse PLTP standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse PLTP standard solution and each sample is measured in duplicate.

    Testpräzision
    • Sample 1: n=16, Mean(ng/ml): 9.4, Standard deviation: 0.498, CV(%): 5.3
    • Sample 2: n=16, Mean(ng/ml): 18.5, Standard deviation: 0.907, CV(%): 4.9
    • Sample 3: n=16, Mean(ng/ml): 27, Standard deviation: 1.134, CV(%): 4.2,
    • Sample 1: n=24, Mean(ng/ml): 11.2, Standard deviation: 0.75, CV(%): 6.7
    • Sample 2: n=24, Mean(ng/ml): 17.9, Standard deviation: 0.931, CV(%): 5.2
    • Sample 3: n=24, Mean(ng/ml): 31, Standard deviation: 1.49, CV(%): 4.8
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    Avoid multiple freeze-thaw cycles.
    Lagerung
    -20 °C,4 °C
    Informationen zur Lagerung
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Haltbarkeit
    12 months
  • Target Alle PLTP ELISA Kits anzeigen
    PLTP (Phospholipid Transfer Protein (PLTP))
    Andere Bezeichnung
    PLTP (PLTP Produkte)
    Synonyme
    zgc:100903 ELISA Kit, MGC84445 ELISA Kit, PLTP ELISA Kit, hdlcq9 ELISA Kit, DKFZp469A1437 ELISA Kit, BPIFE ELISA Kit, HDLCQ9 ELISA Kit, Bpife ELISA Kit, OD107 ELISA Kit, phospholipid transfer protein ELISA Kit, phospholipid transfer protein S homeolog ELISA Kit, PLTP ELISA Kit, pltp ELISA Kit, pltp.S ELISA Kit, LOC542343 ELISA Kit, Pltp ELISA Kit
    Hintergrund

    Protein Function: Facilitates the transfer of a spectrum of different lipid molecules, including diacylglycerol, phosphatidic acid, sphingomyelin, phosphatidylcholine, phosphatidylglycerol, cerebroside and phosphatidyl ethanolamine. Essential for the transfer of excess surface lipids from triglyceride-rich lipoproteins to HDL, thereby facilitating the formation of smaller lipoprotein remnants, contributing to the formation of LDL, and assisting in the maturation of HDL particles. PLTP also plays a key role in the uptake of cholesterol from peripheral cells and tissues that is subsequently transported to the liver for degradation and excretion. Two distinct forms of PLTP exist in plasma: an active form that can transfer PC from phospholipid vesicles to high-density lipoproteins (HDL), and an inactive form that lacks this capability (By similarity). .

    Background: Phospholipid transfer protein (PLTP), also known as lipid transfer protein II is a protein that in humans is encoded by the PLTP gene. This gene is mapped to 20q13.12. The protein encoded by this gene is one of at least two lipid transfer proteins found in human plasma. The encoded protein transfers phospholipids from triglyceride-rich lipoproteins to high density lipoprotein (HDL). In addition to regulating the size of HDL particles, this protein may be involved in cholesterol metabolism. At least two transcript variants encoding different isoforms have been found for this gene.

    Synonyms: Phospholipid transfer protein,Lipid transfer protein II,Pltp,

    Full Gene Name: Phospholipid transfer protein

    Cellular Localisation: Secreted.
    Gen-ID
    18830
    UniProt
    P55065
    Pathways
    Lipid Metabolism
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