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CXCL3 ELISA Kit

CXCL3 Reaktivität: Ratte AA 33-101 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Produktnummer ABIN2859240
  • Target Alle CXCL3 ELISA Kits anzeigen
    CXCL3 (Chemokine (C-X-C Motif) Ligand 3 (CXCL3))
    Bindungsspezifität
    AA 33-101
    Reaktivität
    • 4
    • 3
    • 2
    • 1
    Ratte
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    15.6-1000 pg/mL
    Untere Nachweisgrenze
    15.6 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat CXCL3
    Marke
    PicoKine™
    Proben
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytische Methode
    Quantitative
    Spezifität
    Expression system for standard: E.coli
    Immunogen sequence: R33-S101
    Kreuzreaktivität (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivität
    <10pg/mL
    Benötigtes Material
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: E.coli
    Immunogen sequence: R33-S101
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  • Applikationshinweise
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Plattentyp
    Pre-coated
    Protokoll
    rat CXCL3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CXCL3 has been precoated onto 96-well plates. Standards(E.coli, R33-S101) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat CXCL3 amount of sample captured in plate.
    Testdurchführung

    Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL rat CXCL3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat CXCL3 standard solution and each sample be measured in duplicate.

    Testpräzision
    • Sample 1: n=16, Mean(pg/ml): 170, Standard deviation: 13.26, CV(%): 7.8
    • Sample 2: n=16, Mean(pg/ml): 375, Standard deviation: 19.5, CV(%): 5.2
    • Sample 3: n=16, Mean(pg/ml): 602, Standard deviation: 27.09, CV(%): 4.5,
    • Sample 1: n=24, Mean(pg/ml): 161, Standard deviation: 13.36, CV(%): 8.3
    • Sample 2: n=24, Mean(pg/ml): 352, Standard deviation: 22.53, CV(%): 6.4
    • Sample 3: n=24, Mean(pg/ml): 593, Standard deviation: 27.87, CV(%): 4.7
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    Avoid multiple freeze-thaw cycles.
    Lagerung
    -20 °C,4 °C
    Informationen zur Lagerung
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Haltbarkeit
    12 months
  • Target Alle CXCL3 ELISA Kits anzeigen
    CXCL3 (Chemokine (C-X-C Motif) Ligand 3 (CXCL3))
    Andere Bezeichnung
    CXCL3 (CXCL3 Produkte)
    Synonyme
    CXCL3 ELISA Kit, CINC-2b ELISA Kit, GRO3 ELISA Kit, GROg ELISA Kit, MIP-2b ELISA Kit, MIP2B ELISA Kit, SCYB3 ELISA Kit, Cinc-2 ELISA Kit, Cinc2 ELISA Kit, Gm1960 ELISA Kit, Dcip1 ELISA Kit, C-X-C motif chemokine ligand 3 ELISA Kit, chemokine (C-X-C motif) ligand 3 ELISA Kit, CXCL3 ELISA Kit, Cxcl3 ELISA Kit
    Hintergrund

    Protein Function: Ligand for CXCR2 (By similarity). Has chemotactic activity for neutrophils. May play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. .

    Background: Chemokine (C-X-C motif) ligand 3 (CXCL3) is a small cytokine belonging to the CXC chemokine family. It is mapped to 14p2 in rat. CXCL3 controls migration and adhesion of monocytes and mediates its effects on its target cell by interacting with a cell surface chemokine receptor. It has been shown that CXCL3 regulates cell autonomously the migration of the precursors of cerebellar granule neurons toward the internal layers of cerebellum, during the morphogenesis of cerebellum. CXCL3 also play fundamental roles in the development, homeostasis and it has effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis.

    Synonyms: C-X-C motif chemokine 3,Cytokine-induced neutrophil chemoattractant 2,CINC-2,Macrophage inflammatory protein 2-alpha/beta,MIP2-alpha/beta,Cxcl3,Cinc2,

    Full Gene Name: C-X-C motif chemokine 3

    Cellular Localisation: Secreted.
    Gen-ID
    171551
    UniProt
    Q10746
    Pathways
    Cellular Response to Molecule of Bacterial Origin, Autophagie
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