CXCL3 ELISA Kit

Produktnummer ABIN2859240

Produktdetails CXCL3 ELISA Kit

Target: CXCL3 (Chemokine (C-X-C Motif) Ligand 3 (CXCL3))

Bindungsspezifität: AA 33-101

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.6-1000 pg/mL

Untere Nachweisgrenze: 15.6 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat CXCL3

Marke: PicoKine™

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytische Methode: Quantitative

Spezifität: Expression system for standard: E.coli
Immunogen sequence: R33-S101

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <10pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: E.coli
Immunogen sequence: R33-S101

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plattentyp: Pre-coated

Protokoll: rat CXCL3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CXCL3 has been precoated onto 96-well plates. Standards(E.coli, R33-S101) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat CXCL3 amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL rat CXCL3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat CXCL3 standard solution and each sample be measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 170, Standard deviation: 13.26, CV(%): 7.8
• Sample 2: n=16, Mean(pg/ml): 375, Standard deviation: 19.5, CV(%): 5.2
• Sample 3: n=16, Mean(pg/ml): 602, Standard deviation: 27.09, CV(%): 4.5,
• Sample 1: n=24, Mean(pg/ml): 161, Standard deviation: 13.36, CV(%): 8.3
• Sample 2: n=24, Mean(pg/ml): 352, Standard deviation: 22.53, CV(%): 6.4
• Sample 3: n=24, Mean(pg/ml): 593, Standard deviation: 27.87, CV(%): 4.7

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Details zu CXCL3

Target: CXCL3 (Chemokine (C-X-C Motif) Ligand 3 (CXCL3))

Andere Bezeichnung: CXCL3 (CXCL3 Produkte)

Synonyme: CXCL3 ELISA Kit, CINC-2b ELISA Kit, GRO3 ELISA Kit, GROg ELISA Kit, MIP-2b ELISA Kit, MIP2B ELISA Kit, SCYB3 ELISA Kit, Cinc-2 ELISA Kit, Cinc2 ELISA Kit, Gm1960 ELISA Kit, Dcip1 ELISA Kit, C-X-C motif chemokine ligand 3 ELISA Kit, chemokine (C-X-C motif) ligand 3 ELISA Kit, CXCL3 ELISA Kit, Cxcl3 ELISA Kit

Hintergrund:

Protein Function: Ligand for CXCR2 (By similarity). Has chemotactic activity for neutrophils. May play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. .

Background: Chemokine (C-X-C motif) ligand 3 (CXCL3) is a small cytokine belonging to the CXC chemokine family. It is mapped to 14p2 in rat. CXCL3 controls migration and adhesion of monocytes and mediates its effects on its target cell by interacting with a cell surface chemokine receptor. It has been shown that CXCL3 regulates cell autonomously the migration of the precursors of cerebellar granule neurons toward the internal layers of cerebellum, during the morphogenesis of cerebellum. CXCL3 also play fundamental roles in the development, homeostasis and it has effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis.

Synonyms: C-X-C motif chemokine 3,Cytokine-induced neutrophil chemoattractant 2,CINC-2,Macrophage inflammatory protein 2-alpha/beta,MIP2-alpha/beta,Cxcl3,Cinc2,

Full Gene Name: C-X-C motif chemokine 3

Cellular Localisation: Secreted.

Gen-ID: 171551

UniProt: Q10746

Pathways: Cellular Response to Molecule of Bacterial Origin, Autophagie