ANGPTL3 ELISA Kit

Produktnummer ABIN2859241

Produktdetails ANGPTL3 ELISA Kit

Target: ANGPTL3 (Angiopoietin-Like 3 (ANGPTL3))

Bindungsspezifität: AA 17-460

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 312-20.000 pg/mL

Untere Nachweisgrenze: 312 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human ANGPTL3

Marke: PicoKine™

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytische Methode: Quantitative

Spezifität: Expression system for standard: sf21
Immunogen sequence: S17-E460

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <10pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: sf21
Immunogen sequence: S17-E460

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Sequence similarities: Contains 1 fibrinogen C-terminal domain.

Tissue Specificity: Expressed principally in liver. Weakly expressed in kidney. .

Plattentyp: Pre-coated

Protokoll: human ANGPTL3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ANGPTL3 has been precoated onto 96-well plates. Standards(sf21, S17-E460) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ANGPTL3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human ANGPTL3 amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 20,000pg/mL, 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human ANGPTL3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human ANGPTL3 standard solution and each sample be measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(ng/ml): 3.2, Standard deviation: 0.144, CV(%): 4.5
• Sample 2: n=16, Mean(ng/ml): 5.8, Standard deviation: 0.273, CV(%): 4.7
• Sample 3: n=16, Mean(ng/ml): 10.3, Standard deviation: 0.299, CV(%): 2.9,
• Sample 1: n=24, Mean(ng/ml): 3.5, Standard deviation: 0.238, CV(%): 6.8
• Sample 2: n=24, Mean(ng/ml): 6.2, Standard deviation: 0.459, CV(%): 7.4
• Sample 3: n=24, Mean(ng/ml): 12.1, Standard deviation: 0.762, CV(%): 6.3

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Details zu ANGPTL3

Target: ANGPTL3 (Angiopoietin-Like 3 (ANGPTL3))

Andere Bezeichnung: ANGPTL3 (ANGPTL3 Produkte)

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Hintergrund:

Background: ANGPTL3, also known as Angiopoietin-like 3 or HYPLl, is a protein that in humans is encoded by the ANGPTL3 gene. It is mapped to 1p31.3. The protein encoded by this gene is a member of the angiopoietin-like family of secreted factors. It is expressed predominantly in the liver, and has the characteristic structure of angiopoietins, consisting of a signal peptide, N-terminal coiled-coil domain, and the C-terminal fibrinogen (FBN)-like domain. ANGPTL3 also acts as dual inhibitor of lipoprotein lipase (LPL) and endothelial lipase (EL), and increases plasma triglyceride and HDL cholesterol in rodents. It can inhibit endothelial lipase to catalyze HDL-phospholipid and increase HDL-PL levels.

Synonyms: Angiopoietin-related protein 3,Angiopoietin-5,ANG-5,Angiopoietin-like protein 3,ANGPTL3,ANGPT5,UNQ153/PRO179,

Full Gene Name: Angiopoietin-related protein 3

Cellular Localisation: Secreted.

Gen-ID: 27329

UniProt: Q9Y5C1