FGF21 ELISA Kit

Produktnummer ABIN2859244

Produktdetails FGF21 ELISA Kit

Target: FGF21 (Fibroblast Growth Factor 21 (FGF21))

Bindungsspezifität: AA 29-210

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.2-2000 pg/mL

Untere Nachweisgrenze: 31.2 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse FGF21

Marke: PicoKine™

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytische Methode: Quantitative

Spezifität: E.coli, A29-S210

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <10pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: E.coli
Immunogen sequence: A29-S210

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Tissue Specificity: Most abundantly expressed in the liver, also expressed in the thymus at lower levels. .

Plattentyp: Pre-coated

Protokoll: mouse FGF21 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for FGF21 has been precoated onto 96-well plates. Standards(E.coli,A29-S210) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FGF21 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse FGF21 amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse FGF21 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma (heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse FGF21 standard solution and each sample be measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 245, Standard deviation: 11.51, CV(%): 4.7
• Sample 2: n=16, Mean(pg/ml): 656, Standard deviation: 28.20, CV(%): 4.3
• Sample 3: n=16, Mean(pg/ml): 1422, Standard deviation: 71.1, CV(%): 5.0,
• Sample 1: n=24, Mean(pg/ml): 210, Standard deviation: 12.81, CV(%): 6.1
• Sample 2: n=24, Mean(pg/ml): 670, Standard deviation: 43.55, CV(%): 6.5
• Sample 3: n=24, Mean(pg/ml): 1572, Standard deviation: 99.03, CV(%): 6.3

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Referenzen für FGF21 ELISA Kit (ABIN2859244)

Liu, Pan, Chen, Yang, Zhao, Jia, Zhu, Zhang, Gao, Li, Han, Duan: "Administration of Danhong Injection to diabetic db/db mice inhibits the development of diabetic retinopathy and nephropathy." in: Scientific reports, Vol. 5, pp. 11219, (2015) (PubMed).

Details zu FGF21

Target: FGF21 (Fibroblast Growth Factor 21 (FGF21))

Andere Bezeichnung: FGF21 (FGF21 Produkte)

Synonyme: FGF21 ELISA Kit, fibroblast growth factor 21 ELISA Kit, FGF21 ELISA Kit, fgf21 ELISA Kit, Fgf21 ELISA Kit

Hintergrund:

Protein Function: Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity probably requires the presence of KLB. .

Background: Fibroblast growth factor 21 is a protein that in humans is encoded by the FGF21 gene. By genomic sequence analysis, Nishimura et al. (2000) identified the FGF21 gene within the 5-prime flanking region of the FUT1 gene on chromosome 19. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF21 stimulates glucose uptake in adipocytes but not in other cell types. This effect is additive to the activity of insulin. FGF21 treatment of adipocytes is associated with phosphorylation of FRS2, a protein linking FGF receptors to the Ras/MAP kinase pathway. FGF21 injection in ob/ob mice results in an increase in Glut1 in adipose tissue. FGF21 also protects animals from diet-induced obesity when overexpressed in transgenic mice and lowers blood glucose and triglyceride levels when administered to diabetic rodents.

Synonyms: Fibroblast growth factor 21,FGF-21,Fgf21,

Full Gene Name: Fibroblast growth factor 21

Cellular Localisation: Secreted.

Gen-ID: 56636

UniProt: Q9JJN1

Pathways: RTK Signalweg