CD73 ELISA Kit

Produktnummer ABIN2859267

Produktdetails CD73 ELISA Kit

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Bindungsspezifität: AA 27-547

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.2-2000 pg/mL

Untere Nachweisgrenze: 31.2 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CD73/NT5E

Marke: PicoKine™

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytische Methode: Quantitative

Spezifität: Expression system for standard: CHO
Immunogen sequence: W27-K547

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <10pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: CHO
Immunogen sequence: W27-K547

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Sequence similarities: Belongs to the 5'-nucleotidase family.

Plattentyp: Pre-coated

Protokoll: human CD73 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD73 has been precoated onto 96-well plates. Standards(CHO, W27-K547) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD73 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD73 amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human CD73 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum and plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CD73 standard solution and each sample be measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 132, Standard deviation: 5.94, CV(%): 4.5
• Sample 2: n=16, Mean(pg/ml): 510, Standard deviation: 28.56, CV(%): 5.6
• Sample 3: n=16, Mean(pg/ml): 1427, Standard deviation: 91.33, CV(%): 6.4,
• Sample 1: n=24, Mean(pg/ml): 167, Standard deviation: 8.85, CV(%): 5.3
• Sample 2: n=24, Mean(pg/ml): 624, Standard deviation: 37.44, CV(%): 6
• Sample 3: n=24, Mean(pg/ml): 1553, Standard deviation: 111.82, CV(%): 7.2

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Details zu CD73

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Andere Bezeichnung: NT5E (NT5E Produkte)

Synonyme: CALJA ELISA Kit, CD73 ELISA Kit, E5NT ELISA Kit, NT ELISA Kit, NT5 ELISA Kit, NTE ELISA Kit, eN ELISA Kit, eNT ELISA Kit, zgc:63784 ELISA Kit, NT5E ELISA Kit, MGC114869 ELISA Kit, PSPTO2277 ELISA Kit, 2210401F01Rik ELISA Kit, AI447961 ELISA Kit, Nt5 ELISA Kit, 5'-nucleotidase ecto ELISA Kit, 5'-nucleotidase, ecto (CD73) ELISA Kit, 5'-nucleotidase ELISA Kit, 5' nucleotidase, ecto ELISA Kit, NT5E ELISA Kit, nt5e ELISA Kit, nt5e.S ELISA Kit, PSPTO_2277 ELISA Kit, CBU_1233 ELISA Kit, MCA1322 ELISA Kit, Nt5e ELISA Kit

Hintergrund:

Protein Function: Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities. .

Background: 5'-nucleotidase (5'-NT), also known as ecto-5'-nucleotidase or CD73 (Cluster of Differentiation 73), is an enzyme that in humans is encoded by the NT5E gene. Ecto-5-prime-nucleotidase catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. And the enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. Rareallelic variants are associated with a syndrome of adult-onset calcification of joints and arteries (CALJA) affecting the iliac, femoral, and tibial arteries reducing circulation in the legs and the joints of the hands and feet causing pain.

Synonyms: 5'-nucleotidase,5'-NT,3.1.3.5,Ecto-5'-nucleotidase,CD73,NT5E,NT5, NTE,

Full Gene Name: 5'-nucleotidase

Cellular Localisation: Cell membrane, Lipid-anchor, GPI-anchor.

Gen-ID: 4907

UniProt: P21589

Pathways: Synaptic Membrane, Ribonucleoside Biosynthetic Process