TNFRSF1A ELISA Kit
-
- Target Alle TNFRSF1A ELISA Kits anzeigen
- TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A))
- Bindungsspezifität
- AA 30-212
-
Reaktivität
- Maus
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Detektionsbereich
- 15.6-1000 pg/mL
- Untere Nachweisgrenze
- 15.6 pg/mL
- Applikation
- ELISA
- Verwendungszweck
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse TNFR1
- Marke
- PicoKine™
- Proben
- Cell Culture Supernatant, Serum
- Analytische Methode
- Quantitative
- Spezifität
- NSO, L30-A212
- Kreuzreaktivität (Details)
- There is no detectable cross-reactivity with other relevant proteins.
- Sensitivität
- <10pg/mL
- Benötigtes Material
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- Immunogen
-
Expression system for standard: NSO
Immunogen sequence: L30-A212 - Featured
- Zu unserem meistverkauften TNFRSF1A ELISA Kit
- Top Product
- Discover our top product TNFRSF1A ELISA Kit
-
-
- Applikationshinweise
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- Plattentyp
- Pre-coated
- Protokoll
- mouse TNFR1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TNFR1 has been precoated onto 96-well plates. Standards (NSO, L30-A212) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFR1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse TNFR1 amount of sample captured in plate.
- Testdurchführung
-
Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse TNFR1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse TNFR1 standard solution and each sample be measured in duplicate.
- Testpräzision
-
- Sample 1: n=16, Mean(pg/ml): 46, Standard deviation: 2.7, CV(%): 5.9
- Sample 2: n=16, Mean(pg/ml): 118, Standard deviation: 6.5, CV(%): 5.5
- Sample 3: n=16, Mean(pg/ml): 581, Standard deviation: 27.5, CV(%): 4.7,
- Sample 1: n=24, Mean(pg/ml): 48.3, Standard deviation: 2.5, CV(%): 5.2
- Sample 2: n=24, Mean(pg/ml): 120, Standard deviation: 6, CV(%): 5
- Sample 3: n=24, Mean(pg/ml): 592, Standard deviation: 33.3, CV(%): 5.6
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Handhabung
- Avoid multiple freeze-thaw cycles.
- Lagerung
- -20 °C,4 °C
- Informationen zur Lagerung
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- Haltbarkeit
- 12 months
-
- Target Alle TNFRSF1A ELISA Kits anzeigen
- TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A))
- Andere Bezeichnung
- TNFRSF1A (TNFRSF1A Produkte)
- Synonyme
- CD120a ELISA Kit, FPF ELISA Kit, MS5 ELISA Kit, TBP1 ELISA Kit, TNF-R ELISA Kit, TNF-R-I ELISA Kit, TNF-R55 ELISA Kit, TNFAR ELISA Kit, TNFR1 ELISA Kit, TNFR1-d2 ELISA Kit, TNFR55 ELISA Kit, TNFR60 ELISA Kit, p55 ELISA Kit, p55-R ELISA Kit, p60 ELISA Kit, TNF-R1 ELISA Kit, TNF-alphaR1 ELISA Kit, TNFRI ELISA Kit, TNFRp55 ELISA Kit, TNFalpha-R1 ELISA Kit, Tnfr-2 ELISA Kit, Tnfr1 ELISA Kit, fpf ELISA Kit, tbp1 ELISA Kit, p55-r ELISA Kit, tnf-r ELISA Kit, tnfar ELISA Kit, tnfr1 ELISA Kit, cd120a ELISA Kit, tnfr55 ELISA Kit, tnfr60 ELISA Kit, xtnfr1 ELISA Kit, wu:fj65b10 ELISA Kit, zgc:85914 ELISA Kit, TNFR I ELISA Kit, TNFRSF1A ELISA Kit, TNFA ELISA Kit, cTNF ELISA Kit, TNF receptor superfamily member 1A ELISA Kit, tumor necrosis factor receptor superfamily, member 1a ELISA Kit, TNF receptor superfamily member 1A S homeolog ELISA Kit, tumor necrosis factor ELISA Kit, TNFRSF1A ELISA Kit, Tnfrsf1a ELISA Kit, tnfrsf1a.S ELISA Kit, tnfrsf1a ELISA Kit, TNF ELISA Kit
- Hintergrund
-
Protein Function: Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate- specific cysteine proteases) mediating apoptosis (By similarity). .
Background: Tumor necrosis factor receptor 1 (TNFR1), also known as tumor necrosis factor receptor superfamily member 1A (TNFRSF1A) and CD120a, is a ubiquitous membrane receptor that binds tumor necrosis factor-alpha (TNF alpha). The protein encoded by this gene is a member of the tumor necrosis factor receptor superfamily, which also contains TNFRSF1B. And this protein is one of the major receptors for the tumor necrosis factor-alpha. This receptor can activate the transcription factor NF-i°B, mediate apoptosis, and function as a regulator of inflammation. Antiapoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with this receptor, and thus play regulatory roles in the signal transduction mediated by the receptor.
Synonyms: Tumor necrosis factor receptor superfamily member 1A,Tumor necrosis factor receptor 1,TNF-R1,Tumor necrosis factor receptor type I,TNF-RI,TNFR-I,p55,p60,CD120a,Tnfrsf1a,Tnfr-1, Tnfr1,
Full Gene Name: Tumor necrosis factor receptor superfamily member 1A
Cellular Localisation: Cell membrane, Single-pass type I membrane protein. Golgi apparatus membrane, Single-pass type I membrane protein. - Gen-ID
- 21937
- UniProt
- P25118
- Pathways
- NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, Hepatitis C, Ubiquitin Proteasome Pathway
-