IL17D ELISA Kit

Produktnummer ABIN2859299

Produktdetails IL17D ELISA Kit

Target: IL17D (Interleukin 17D (IL17D))

Bindungsspezifität: AA 25-205

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 62.5-4000 pg/mL

Untere Nachweisgrenze: 62.5 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse IL-17D

Marke: PicoKine™

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytische Methode: Quantitative

Spezifität: Expression system for standard: E.coli
Immunogen sequence: A25-R205

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <10pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: E.coli
Immunogen sequence: A25-R205

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plattentyp: Pre-coated

Protokoll: mouse IL-17D ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-17D has been precoated onto 96-well plates. Standards(E.coli, A25-R205) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-17D is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-17D amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse IL-17D standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse IL-17D standard solution and each sample be measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 715, Standard deviation: 30.03, CV(%): 4.2
• Sample 2: n=16, Mean(pg/ml): 1410, Standard deviation: 64.86, CV(%): 4.6
• Sample 3: n=16, Mean(pg/ml): 2703, Standard deviation: 137.9, CV(%): 5.1,
• Sample 1: n=24, Mean(pg/ml): 694, Standard deviation: 38.17, CV(%): 5.5
• Sample 2: n=24, Mean(pg/ml): 1582, Standard deviation: 99.67, CV(%): 6.3
• Sample 3: n=24, Mean(pg/ml): 2691, Standard deviation: 180.3, CV(%): 6.7

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Details zu IL17D

Target: IL17D (Interleukin 17D (IL17D))

Andere Bezeichnung: IL17D (IL17D Produkte)

Synonyme: IL-17D ELISA Kit, IL-22 ELISA Kit, IL-27 ELISA Kit, IL27 ELISA Kit, AI462269 ELISA Kit, Il27a ELISA Kit, il17-4 ELISA Kit, zgc:194257 ELISA Kit, zgc:194303 ELISA Kit, IL-27p28 ELISA Kit, Il30 ELISA Kit, p28 ELISA Kit, interleukin 17D ELISA Kit, interleukin 17d ELISA Kit, interleukin 27 ELISA Kit, IL17D ELISA Kit, Il17d ELISA Kit, il17d ELISA Kit, Il27 ELISA Kit

Hintergrund:

Background: Interleukin 17D (IL-17D) is a member of the unique IL-17 family, which share a conserved cysteine motif. It is mapped to 13q12.11. IL-17D regulates cytokine production in endothelial cells and inhibits hemopoiesis in vitro. Purified recombinant IL-17D inhibited myeloid progenitor cell colony formation. IL-17D can enhance endothelial cell production of IL6, IL8, and GMCSF, but not other cytokines. And it is preferentially expressed in skeletal muscle, brain, adipose tissue, heart, lung, and pancreas.

Synonyms: IL-17D ,Il17d ,

Full Gene Name: interleukin 17D

Gen-ID: 239114

UniProt: Q8K4C4

Pathways: Cellular Response to Molecule of Bacterial Origin