Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

XCL1 ELISA Kit

XCL1 Reaktivität: Maus AA 22-114 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Produktnummer ABIN2859301
  • Target Alle XCL1 ELISA Kits anzeigen
    XCL1 (Chemokine (C Motif) Ligand 1 (XCL1))
    Bindungsspezifität
    AA 22-114
    Reaktivität
    • 5
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Maus
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    62.5-4000 pg/mL
    Untere Nachweisgrenze
    62.5 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse XCL1/Lymphotactin
    Marke
    PicoKine™
    Proben
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytische Methode
    Quantitative
    Spezifität
    E.coli, V22-G114
    Kreuzreaktivität (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivität
    <5pg/mL
    Benötigtes Material
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: E.coli
    Immunogen sequence: V22-G114
    Featured
    Zu unserem meistverkauften XCL1 ELISA Kit
    Top Product
    Discover our top product XCL1 ELISA Kit
  • Applikationshinweise
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Plattentyp
    Pre-coated
    Protokoll
    mouse XCL1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for XCL1 has been precoated onto 96-well plates. Standards (E. coli, V22-G114) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for XCL1is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse XCL1 amount of sample captured in plate.
    Testdurchführung

    Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse XCL1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse XCL1 standard solution and each sample be measured in duplicate.

    Testpräzision
    • Sample 1: n=16, Mean(pg/ml): 447, Standard deviation: 28.61, CV(%): 6.4
    • Sample 2: n=16, Mean(pg/ml): 1253, Standard deviation: 53.88, CV(%): 4.3
    • Sample 3: n=16, Mean(pg/ml): 2346, Standard deviation: 115, CV(%): 4.9,
    • Sample 1: n=24, Mean(pg/ml): 450, Standard deviation: 39.15 , CV(%): 8.7
    • Sample 2: n=24, Mean(pg/ml): 1238, Standard deviation: 64.38 , CV(%): 5.2
    • Sample 3: n=24, Mean(pg/ml): 2625, Standard deviation: 144.4, CV(%): 5.5
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    Avoid multiple freeze-thaw cycles.
    Lagerung
    -20 °C,4 °C
    Informationen zur Lagerung
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Haltbarkeit
    12 months
  • Target Alle XCL1 ELISA Kits anzeigen
    XCL1 (Chemokine (C Motif) Ligand 1 (XCL1))
    Andere Bezeichnung
    XCL1 (XCL1 Produkte)
    Synonyme
    ATAC ELISA Kit, LPTN ELISA Kit, LTN ELISA Kit, SCM-1 ELISA Kit, SCM-1a ELISA Kit, SCM1 ELISA Kit, SCM1A ELISA Kit, SCYC1 ELISA Kit, AI661682 ELISA Kit, Lptn ELISA Kit, Scyc1 ELISA Kit, Ltn ELISA Kit, XCL1 ELISA Kit, XCL2 ELISA Kit, X-C motif chemokine ligand 1 ELISA Kit, chemokine (C motif) ligand 1 ELISA Kit, cytokine SCM-1 beta ELISA Kit, lymphotactin ELISA Kit, XCL1 ELISA Kit, Xcl1 ELISA Kit, LOC490356 ELISA Kit, LOC100714756 ELISA Kit, LOC100349255 ELISA Kit
    Hintergrund

    Protein Function: Chemotactic activity for lymphocytes but not for monocytes or neutrophils.

    Background: Chemokine(C motif) ligand(XCL1) is a small cytokine belonging to the XC chemokine family that is also known as lymphotactin. This gene is mapped to chromosome 1q23 by FISH. The sequence of the deduced 114-amino acid protein is most homologous to the CC chemokines CCL8 and CCL3. It is found in high levels in spleen, thymus, intestine and peripheral blood leukocytes, and at lower levels in lung, prostate gland and ovary. XCL1 induces the migration of cells expressing XCR1.

    Synonyms: Lymphotactin,C motif chemokine 1,Cytokine SCM-1,Lymphotaxin,Small-inducible cytokine C1,Xcl1,Lptn, Ltn, Scyc1,

    Full Gene Name: Lymphotactin

    Cellular Localisation: Secreted.
    Gen-ID
    16963
    UniProt
    P47993
    Pathways
    Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation
Sie sind hier:
Kundenservice