CD166 ELISA Kit

Produktnummer ABIN2859320

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Maus CD166.

Kurzübersicht für CD166 ELISA Kit (ABIN2859320)

Target: CD166 (ALCAM) (Activated Leukocyte Cell Adhesion Molecule (ALCAM))

Bindungsspezifität: AA 28-527

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 156-10.000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Produktdetails CD166 ELISA Kit

Untere Nachweisgrenze: 156 pg/mL

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse ALCAM

Marke: PicoKine™

Analytische Methode: Quantitative

Spezifität: NSO, W28-K527

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <10pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: W28-K527

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Tissue Specificity: Detected on brain motor neurons, in differentiating retinal ganglion cells and in adult retina (PubMed:15345243). Detected on leukocytes and on lymphatic endothelial cells (PubMed:23169771). Detected in spleen B cells and T-cells (at protein level) (PubMed:9209500). Detected in adult brain and embryonic spinal cord (PubMed:15345243). Expressed at high levels in the brain, and lung, and at lower levels in the liver, and the kidney, as well as by activated leukocytes (PubMed:9209500). .

Plattentyp: Pre-coated

Protokoll: mouse ALCAM ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for ALCAM has been precoated onto 96-well plates. Standards(NSO, W28-K527) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ALCAM is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse ALCAM amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse ALCAM standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse ALCAM standard solution and each sample be measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(ng/ml): 0.57, Standard deviation: 0.029, CV(%): 5.1
• Sample 2: n=16, Mean(ng/ml): 2.33, Standard deviation: 0.086, CV(%): 3.7
• Sample 3: n=16, Mean(ng/ml): 4.25, Standard deviation: 0.136, CV(%): 3.2,
• Sample 1: n=24, Mean(ng/ml): 0.68, Standard deviation: 0.058, CV(%): 8.6
• Sample 2: n=24, Mean(ng/ml): 2.74, Standard deviation: 0.17, CV(%): 6.2
• Sample 3: n=24, Mean(ng/ml): 4.61, Standard deviation: 0.244, CV(%): 5.3

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Details zu CD166

Target: CD166 (ALCAM) (Activated Leukocyte Cell Adhesion Molecule (ALCAM))

Andere Bezeichnung: ALCAM

Hintergrund:

Protein Function: Cell adhesion molecule that mediates both heterotypic cell-cell contacts via its interaction with CD6, as well as homotypic cell-cell contacts. Promotes T-cell activation and proliferation via its interactions with CD6 (By similarity). Contributes to the formation and maturation of the immunological synapse via its interactions with CD6 (By similarity). Mediates homotypic interactions with cells that express ALCAM (PubMed:24740813). Mediates attachment of dendritic cells onto endothelial cells via homotypic interaction. Inhibits endothelial cell migration and promotes endothelial tube formation via homotypic interactions (PubMed:23169771). Required for normal organization of the lymph vessel network (PubMed:23169771). Required for normal hematopoietic stem cell engraftment in the bone marrow (PubMed:24740813). Plays a role in hematopoiesis, required for normal numbers of hematopoietic stem cells in bone marrow (PubMed:25730656). Promotes in vitro osteoblast proliferation and differentiation (PubMed:25730656). Promotes neurite extension, axon growth and axon guidance, axons grow preferentially on surfaces that contain ALCAM (By similarity). Mediates outgrowth and pathfinding for retinal ganglion cell axons (PubMed:15345243). .

Background: CD166 antigen is a 100-105 kD typeI transmembrane glycoprotein that is a member of the immunoglobulin superfamily of proteins. In humans it is encoded by the ALCAM gene. The gene was mapped to human chromosome 3q13.1-q13.2 by fluorescence in situ hybridization of cDNA probes to metaphase chromosomes. It is also called CD166 (cluster of differentiation 166), MEMD, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. It is expressed on activated T cells, activated monocytes, epithelial cells, fibroblasts, neurons, melanoma cells, and also in sweat and sebaceous glands. CD166 protein expression is reported to be upregulated in a cell line deriving from a metastasizing melanoma. CD166 plays an important role in mediating adhesion interactions between thymic epithelial cells and CD6+ cells during intrathymic T cell development. Recently, CD166 has also been used as a potential cancer stem cell marker.

Synonyms: CD166 antigen,Activated leukocyte cell adhesion molecule ,BEN ,Protein DM-GRASP ,CD166,Alcam,

Full Gene Name: CD166 antigen

Cellular Localisation: Cell membrane, Single-pass type I membrane protein . Cell projection, axon . Cell projection, dendrite . Detected at the immunological synapse, i.e, at the contact zone between antigen-presenting dendritic cells and T-cells. Colocalizes with CD6 and the TCR/CD3 complex at the immunological synapse..

Gen-ID: 11658

UniProt: Q61490