Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

C-JUN ELISA Kit

JUN Reaktivität: Human Colorimetric DNA-Binding ELISA Cell Extracts, Tissue Samples
Produktnummer ABIN2866255
  • Target Alle C-JUN (JUN) ELISA Kits anzeigen
    C-JUN (JUN) (Jun Proto-Oncogene (JUN))
    Reaktivität
    • 23
    • 19
    • 17
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    DNA-Binding ELISA
    Applikation
    ELISA
    Verwendungszweck
    DNA-binding ELISA that facilitate the study of transcription factor activation in mammalien tissue and cell culture extracts.
    Marke
    TransAM®
    Proben
    Cell Extracts, Tissue Samples
    Analytische Methode
    Quantitative
    Spezifität
    TransAM AP-1 c-Jun Kits are tested for sensitivity in detecting c-Jun in nuclear extracts from K-562 or WI-38 VA13 cells that are either unstimulated or stimulated with TPA or TPA/Ionomycin.
    Produktmerkmale
    Transcription factors are DNA-binding proteins that tightly regulate gene expression. They consist of two distinct domains - one that displays high affinity for a specific DNA sequence and one that confers transcriptional activity. Transcription factors are activated by phosphorylation of specific residues or by processing bound inhibitory proteins. Understanding and quantifying transcription factors is essential for the study of cell functions in relation to differentiation, brain activity, immune response, inflammation and various disease states. TransAM® Kits are sensitive, non-radioactive transcription factor ELISA kits that facilitate the study of transcription factor activation in mammalian tissue and cell extracts.
    TransAM® Kits are DNA-binding ELISAs that facilitate the study of transcription factor activation in mammalian tissue and cell extracts. Each kit includes a 96-stripwell plate in which multiple copies of a specific double-stranded oligonucleotide have been immobilized. When nuclear or whole-cell extract is added, activated transcription factor of interest binds the oligonucleotide at its consensus binding site and is quantified using the included antibody, which is specific for the bound, active form of the transcription factor being studied.
    Bestandteile
    One or five 96-well plate(s) with plate sealer(s), primary antibody, HRP-conjugated secondary antibody, wild-type and mutated competitor oligonucleotides, positive control cell extract, DTT, Protease Inhibitor Cocktail, Lysis, Binding, 10X Washing and 10X Antibody Binding Buffers, and Developing and Stop Solutions.
    Featured
    Zu unserem meistverkauften JUN ELISA Kit
    Top Product
    Discover our top product JUN ELISA Kit
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Kommentare

    These extracts are diluted down to 0.625 μg/well and assayed using the TransAM AP-1 c-Jun Kit. The ratio of the signals from the stimulated over the unstimulated cells must be above 2. Lot No.18715036 was tested using K-562 cells. It was developed for 4 minutes and gave a ratio of 6.4 (Figure 1). The basal level of c-Jun expression, and this ratio, may vary depending on the cell type tested and the stimulation used. TransAM AP-1 c-Jun Kits are also tested for specificity in detecting c-Jun activation. TransAM AP-1 c-Jun assays are performed in the presence of an excess of oligonucleotide containing a wild-type or mutated AP-1 consensus binding site (Figure 2). At 20X excess, the wild-type oligonucleotide prevents AP-1 binding to the probe immobilized on the plate. Conversely, the mutated oligonucleotide has little effect on AP-1 binding.

    Testdauer
    5 h
    Plattentyp
    Pre-coated
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    4 °C/-20 °C/-80 °C
    Informationen zur Lagerung
    Except for the cell extract that must be kept at -80°C, the kit components can be stored at -20°C prior to first use. Then, we recommend storing the kit at 4°C except for the phospho-c-Jun antibody,DTT, oligonucleotides, poly[d(I-C)] and Protease Inhibitor Cocktail that should be kept at -20°C, and the cell extract at -80°C. This product is guaranteed for 6 months from date of receipt.
    Haltbarkeit
    6 months
  • Target Alle C-JUN (JUN) ELISA Kits anzeigen
    C-JUN (JUN) (Jun Proto-Oncogene (JUN))
    Andere Bezeichnung
    Ap-1 C-Jun (JUN Produkte)
    Synonyme
    AP-1 ELISA Kit, AP1 ELISA Kit, c-Jun ELISA Kit, fj36h07 ELISA Kit, wu:fj36h07 ELISA Kit, zgc:65863 ELISA Kit, Junc ELISA Kit, c-jun ELISA Kit, CG2275 ELISA Kit, D-Jun ELISA Kit, D-jun ELISA Kit, D-jun/Jra ELISA Kit, DJUN ELISA Kit, DJun ELISA Kit, Djun ELISA Kit, Dmel\\CG2275 ELISA Kit, JRA ELISA Kit, JUN ELISA Kit, Jun ELISA Kit, V ELISA Kit, cJun ELISA Kit, d-JRA ELISA Kit, d-Jun ELISA Kit, d-jun ELISA Kit, dAP-1 ELISA Kit, dJRA ELISA Kit, dJUN ELISA Kit, dJra ELISA Kit, dJun ELISA Kit, djun ELISA Kit, dm-Jun ELISA Kit, jra ELISA Kit, jun ELISA Kit, l(2)46Ef ELISA Kit, l(2)IA109 ELISA Kit, l(2R)IA109 ELISA Kit, Jun proto-oncogene, AP-1 transcription factor subunit ELISA Kit, jun proto-oncogene S homeolog ELISA Kit, jun proto-oncogene ELISA Kit, Jun-related antigen ELISA Kit, jun proto-oncogene L homeolog ELISA Kit, JUN ELISA Kit, Jun ELISA Kit, jun.S ELISA Kit, jun ELISA Kit, Jra ELISA Kit, jun.L ELISA Kit
    Pathways
    MAPK Signalweg, RTK Signalweg, WNT Signalweg, Fc-epsilon Rezeptor Signalübertragung, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Autophagie, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling, S100 Proteine
Sie sind hier:
Kundenservice