FOXO1 ELISA Kit

Produktnummer ABIN2866265

Produktdetails FOXO1 ELISA Kit

Target: FOXO1 (Forkhead Box O1 (FOXO1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: DNA-Binding ELISA

Applikation: ELISA

Verwendungszweck: DNA-binding ELISA that facilitate the study of transcription factor activation in mammalien tissue and cell culture extracts.

Marke: TransAM®

Proben: Cell Extracts, Tissue Samples

Analytische Methode: Quantitative

Spezifität: TransAM FKHR (FOXO1/4) Kits are tested for sensitivity in detecting FKHR activation.

Produktmerkmale: Transcription factors are DNA-binding proteins that tightly regulate gene expression. They consist of two distinct domains - one that displays high affinity for a specific DNA sequence and one that confers transcriptional activity. Transcription factors are activated by phosphorylation of specific residues or by processing bound inhibitory proteins. Understanding and quantifying transcription factors is essential for the study of cell functions in relation to differentiation, brain activity, immune response, inflammation and various disease states. TransAM® Kits are sensitive, non-radioactive transcription factor ELISA kits that facilitate the study of transcription factor activation in mammalian tissue and cell extracts.
TransAM® Kits are DNA-binding ELISAs that facilitate the study of transcription factor activation in mammalian tissue and cell extracts. Each kit includes a 96-stripwell plate in which multiple copies of a specific double-stranded oligonucleotide have been immobilized. When nuclear or whole-cell extract is added, activated transcription factor of interest binds the oligonucleotide at its consensus binding site and is quantified using the included antibody, which is specific for the bound, active form of the transcription factor being studied.

Bestandteile: One or five 96-well plate(s) with plate sealer(s), primary antibody, HRP-conjugated secondary antibody, wild-type and mutated competitor oligonucleotides, positive control cell extract, DTT, Protease Inhibitor Cocktail, Lysis, Binding, 10X Washing and 10X Antibody Binding Buffers, and Developing and Stop Solutions.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Nuclear extracts prepared from Raji and HeLa cells are diluted to 1.2 μg/well and assayed using the TransAM FKHR (FOXO1/4) Kit. The ratio of the signals from the Raji cells over the HeLa cells must be above 5. Lot No. 09814003 was developed for 12 minutes. It gave a ratio of 5.72(Figure 1). The endogenous level of FKHR expression, and this ratio may vary depending on the cell type tested and the treatment used. TransAM FKHR (FOXO1/4) Kits are also tested for specificity in detecting FKHR (FOXO1/4) activity. TransAM FKHR (FOXO1/4) assays are performed in the presence of an excess of oligonucleotide containing a wild-type or mutated FKHR consensus binding site (Figure 2). At 10X excess, the wild-type oligonucleotide prevents FKHR binding to the probe immobilized on the plate. Conversely, the mutated oligonucleotide has no effect.

Testdauer: 5 h

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C/-80 °C

Informationen zur Lagerung: Store the cell extract at -80°C. Other kit components can be stored at -20°C prior to first use. Then, we recommend storing the kit at 4°C except for the primary antibody, oligonucleotides, DTT and Protease Inhibitor Cocktail that should be kept at -20°C. This product is guaranteed for 6 months from date of receipt.

Haltbarkeit: 6 months

Details zu FOXO1

Target: FOXO1 (Forkhead Box O1 (FOXO1))

Andere Bezeichnung: Fkhr (FOXO1 Produkte)

Synonyme: FKH1 ELISA Kit, FKHR ELISA Kit, FOXO1A ELISA Kit, FoxO1a.1 ELISA Kit, foxo1 ELISA Kit, zgc:153388 ELISA Kit, Fkhr ELISA Kit, Foxo1a ELISA Kit, AI876417 ELISA Kit, Afxh ELISA Kit, Fkhr1 ELISA Kit, fkh1 ELISA Kit, fkhr ELISA Kit, foxo1a ELISA Kit, xFoxO1 ELISA Kit, FoxO1A ELISA Kit, foxo1a.2 ELISA Kit, forkhead box O1 ELISA Kit, forkhead box O1 a ELISA Kit, forkhead box O1 L homeolog ELISA Kit, forkhead box O1 b ELISA Kit, FOXO1 ELISA Kit, foxo1a ELISA Kit, Foxo1 ELISA Kit, foxo1 ELISA Kit, foxo1.L ELISA Kit, foxo1b ELISA Kit

Pathways: PI3K-Akt Signalweg, Zellzyklus, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Carbohydrate Homeostasis, Chromatin Binding, Regulation of Carbohydrate Metabolic Process, CXCR4-mediated Signaling Events, BCR Signaling