Endomorphin 1 FLISA Kit

Produktnummer ABIN2871394

Produktdetails Endomorphin 1 FLISA Kit

Target: Endomorphin 1 (EM1)

Reaktivität: Human

Nachweismethode: Fluorometric

Methodentyp: Competition ELISA

Detektionsbereich: 29.7-940 pg/mL

Untere Nachweisgrenze: 29.7 pg/mL

Applikation: ELISA

Analytische Methode: Quantitative

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll: The immunoplate in this kit is pre-coated with a secondary antibody and the nonspecific binding sites are blocked. The secondary antibody can bind to the Fc fragment of the primary antibody (peptide antibody) whose Fab fragment will be competitively bound by both biotinylated peptide and peptide standard or targeted peptide in samples. The biotinylated peptide interacts with streptavidin-horseradish peroxidase (SA-HRP) which catalyzes the substrate. The fluorescence intensity is directly proportional to the amount of biotinylated peptide-SA-HRP complex but inversely proportional to the amount of the peptide in standard solutions or samples. This is due to the competitive binding of the biotinylated peptide with the standard peptide or samples to the peptide antibody (primary antibody). A standard curve of known concentration can be established accordingly. The unknown concentration in samples can be determined by extrapolation to this standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Antigendetails

Target: Endomorphin 1 (EM1)

Andere Bezeichnung: Endomorphin-1 (EM1 Produkte)