IDS ELISA Kit
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- Target Alle IDS ELISA Kits anzeigen
- IDS (Iduronate 2-Sulfatase (IDS))
- Bindungsspezifität
- AA 36-552
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Reaktivität
- Maus
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Detektionsbereich
- 156-10000 pg/mL
- Untere Nachweisgrenze
- 156 pg/mL
- Applikation
- ELISA
- Verwendungszweck
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse IDS
- Marke
- PicoKine™
- Proben
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytische Methode
- Quantitative
- Spezifität
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Expression system for standard: NSO
Immunogen sequence: T36-P552 - Kreuzreaktivität (Details)
- There is no detectable cross-reactivity with other relevant proteins.
- Sensitivität
- <15pg/mL
- Benötigtes Material
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- Immunogen
- Immunogen sequence: T36-P552
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- Applikationshinweise
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- Kommentare
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Tissue Specificity: Found to be expressed in alpha and beta pancreatic cells. .
- Plattentyp
- Pre-coated
- Protokoll
- mouse IDS ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IDS has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: T36-P552) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IDS is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IDS amount of sample captured in plate.
- Testdurchführung
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Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse IDS standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse IDS standard solution and each sample be measured in duplicate.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Buffer
- heparin or EDTA
- Handhabung
- Avoid multiple freeze-thaw cycles.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- Haltbarkeit
- 12 months
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- Target Alle IDS ELISA Kits anzeigen
- IDS (Iduronate 2-Sulfatase (IDS))
- Andere Bezeichnung
- IDS (IDS Produkte)
- Synonyme
- mps2 ELISA Kit, sids ELISA Kit, zgc:158245 ELISA Kit, MPS2 ELISA Kit, SIDS ELISA Kit, AW214631 ELISA Kit, iduronate 2-sulfatase ELISA Kit, IDS ELISA Kit, CpipJ_CPIJ004938 ELISA Kit, ids ELISA Kit, Ids ELISA Kit
- Hintergrund
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Protein Function: Required for the lysosomal degradation of heparan sulfate and dermatan sulfate. .
Background: Iduronate 2-sulfatase (IDS) is a sulfatase enzyme associated with Hunter syndrome. It encodes a member of the sulfatase family of proteins. Iduronate 2-sulfatase is involved in the lysosomal degradation of the glycosaminoglycans heparan sulfate and dermatan sulfate. The encoded preproprotein is proteolytically processed to generate two polypeptide chains. Mutations in this gene are associated with the X-linked lysosomal storage disease mucopolysaccharidosis type II, also known as Hunter syndrome. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed.
Synonyms: Iduronate 2-sulfatase,3.1.6.13,Alpha-L-iduronate sulfate sulfatase,Ids,
Full Gene Name: Iduronate 2-sulfatase
Cellular Localisation: Lysosome. - Gen-ID
- 15931
- UniProt
- Q08890
- Pathways
- Glycosaminoglycan Metabolic Process
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