Interferon gamma ELISA Kit

Produktnummer ABIN411283

Produktdetails Interferon gamma ELISA Kit

Target: Interferon gamma (IFNG)

Bindungsspezifität: AA 23-155

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.2-2000 pg/mL

Untere Nachweisgrenze: 31.2 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse IFN gamma

Marke: PicoKine™

Proben: Cell Culture Supernatant, Serum

Analytische Methode: Quantitative

Spezifität: Expression system for standard: E.coli
Immunogen sequence: H23-C155

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <5pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: E.coli
Immunogen sequence: H23-C155

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Tissue Specificity: Released primarily from activated T lymphocytes.

Plattentyp: Pre-coated

Protokoll: mouse IFN gamma ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IFN gamma has been precoated onto 96-well plates. Standards(E.coli, H23-C155) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IFN gamma is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IFN gamma amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse IFN gamma standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse IFN gamma standard solution and each sample be measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 305, Standard deviation: 11.59, CV(%): 3.8
• Sample 2: n=16, Mean(pg/ml): 782, Standard deviation: 35.19, CV(%): 4.5
• Sample 3: n=16, Mean(pg/ml): 1367, Standard deviation: 69.72, CV(%): 5.1,
• Sample 1: n=24, Mean(pg/ml): 350, Standard deviation: 19.6, CV(%): 5.6
• Sample 2: n=24, Mean(pg/ml): 812, Standard deviation: 55.22, CV(%): 6.8
• Sample 3: n=24, Mean(pg/ml): 1494, Standard deviation: 109.1, CV(%): 7.3

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Referenzen für Interferon gamma ELISA Kit (ABIN411283)

Qin, Gao, Liu, Liu, Wang, Zou: "Silencing of suppressor of cytokine signaling 1 enhances the immunological effect of mucin 1-calreticulin-primed 4T1 cell-treated dendritic cells in breast cancer treatment." in: Oncology letters, Vol. 15, Issue 2, pp. 1630-1638, (2018) (PubMed).

Lu, Liu, Jin, Chen, Liang, Qiu, Dai: "Herbal Components of a Novel Formula PSORI-CM02 Interdependently Suppress Allograft Rejection and Induce CD8+CD122+PD-1+ Regulatory T Cells." in: Frontiers in pharmacology, Vol. 9, pp. 88, (2018) (PubMed).

Hu, Feng, Wang, Song, Ni: "5-BDBD ameliorates an OVA-induced allergic asthma by the reduction of Th2 cytokines production." in: Iranian journal of basic medical sciences, Vol. 21, Issue 4, pp. 364-369, (2018) (PubMed).

Zhang, Li, Wang, Xie, Li, Zuo, Kong, Wang, Wang: "Immune Protection of Rhoptry Protein 21 (ROP21) of Toxoplasma gondii as a DNA Vaccine Against Toxoplasmosis." in: Frontiers in microbiology, Vol. 9, pp. 909, (2018) (PubMed).

Xu, Liu, Wang, Li, Wu, Li, Di, You, Jiang et al.: "3-(1H-Benzo[d]imidazol-6-yl)-5-(4-fluorophenyl)-1,2,4-oxadiazole (DDO7232), a Novel Potent Nrf2/ARE Inducer, Ameliorates DSS-Induced Murine Colitis and Protects NCM460 Cells against Oxidative Stress ..." in: Oxidative medicine and cellular longevity, Vol. 2018, pp. 3271617, (2018) (PubMed).

Liu, Huang, Yao, Yang: "The Immunotherapeutic Role of Bacterial Lysates in a Mouse Model of Asthma." in: Lung, (2017) (PubMed).

He, Liu, Xu: "The cryo-thermal therapy eradicated melanoma in mice by eliciting CD4(+) T-cell-mediated antitumor memory immune response." in: Cell death & disease, Vol. 8, Issue 3, pp. e2703, (2017) (PubMed).

Wang, Zhang, Wang, Gadahi, Xu, Yan, Song, Li: "Toxoplasma gondii Elongation Factor 1-Alpha (TgEF-1α) Is a Novel Vaccine Candidate Antigen against Toxoplasmosis." in: Frontiers in microbiology, Vol. 8, pp. 168, (2017) (PubMed).

Liang, Song, Liang, Zhang, Gao, Fan: "The hemagglutinin-neuramidinase protein of Newcastle disease virus upregulates expression of the TRAIL gene in murine natural killer cells through the activation of Syk and NF-κB." in: PLoS ONE, Vol. 12, Issue 6, pp. e0178746, (2017) (PubMed).

Xu, Zhang, Li, Liu, Tang, Fan: "hsdS, Belonging to the Type I Restriction-Modification System, Contributes to the Streptococcus suis Serotype 2 Survival Ability in Phagocytes." in: Frontiers in microbiology, Vol. 8, pp. 1524, (2017) (PubMed).

Wang, Zhang, Xi, Huang, Nie, Zhou, Deng, Tao: "Establishment of a mouse model of lipopolysaccharide-induced neutrophilic nasal polyps." in: Experimental and therapeutic medicine, Vol. 14, Issue 6, pp. 5275-5282, (2017) (PubMed).

Wang, Su, Dong, Shi, Gao, Wang: "Hybrid phage displaying SLAQVKYTSASSI induces protection against Candida albicans challenge in BALB/c mice." in: Human vaccines & immunotherapeutics, Vol. 10, Issue 4, pp. 1057-63, (2016) (PubMed).

Wu, Wen, Xiang, Huang, Gao, Qu: "Immunostimulative Activity of Low Molecular Weight Chitosans in RAW264.7 Macrophages." in: Marine drugs, Vol. 13, Issue 10, pp. 6210-25, (2016) (PubMed).

Wang, Wang, Sun, Zhang, Liu, Gadahi, Hassan, Xu, Yan, Song, Li: "Protective immunity against acute toxoplasmosis in BALB/c mice induced by a DNA vaccine encoding Toxoplasma gondii elongation factor 1-alpha." in: BMC infectious diseases, Vol. 15, pp. 448, (2016) (PubMed).

Wang, Ji, Zhang, Fan, Zhang, Shi, Zhou, Chen, Wang, Wang: "Stimulation of dendritic cells by DAMPs in ALA-PDT treated SCC tumor cells." in: Oncotarget, Vol. 6, Issue 42, pp. 44688-702, (2016) (PubMed).

Chen, Zhang, Sun: "Immunological adjuvant effect of the peptide fraction from the larvae of Musca domestica." in: BMC complementary and alternative medicine, Vol. 15, Issue 1, pp. 427, (2016) (PubMed).

Qin, Lu, Guo, Luo, Hu, Huang, Chen, Wang, Ma, Yang, Jiang, Zhou: "A deleterious role for Th9/IL-9 in hepatic fibrogenesis." in: Scientific reports, Vol. 6, pp. 18694, (2016) (PubMed).

Huai, Dong, Zhu, Li, Cao, Gao, Yang, Wang, Mao: "Genetically Engineered Virus Nanofibers as an Efficient Vaccine for Preventing Fungal Infection." in: Advanced healthcare materials, Vol. 5, Issue 7, pp. 786-94, (2016) (PubMed).

Hassan, Wang, Xu, Yan, Song, Li: "DNA vaccination with a gene encoding Toxoplasma gondii Deoxyribose Phosphate Aldolase (TgDPA) induces partial protective immunity against lethal challenge in mice." in: Parasites & vectors, Vol. 7, pp. 431, (2015) (PubMed).

Zhang, Qian, Liu, Qian, Chen, Li: "Virus-like particles of chimeric recombinant porcine circovirus type 2 as antigen vehicle carrying foreign epitopes." in: Viruses, Vol. 6, Issue 12, pp. 4839-55, (2015) (PubMed).

Details zu Interferon gamma

Target: Interferon gamma (IFNG)

Andere Bezeichnung: IFNG (IFNG Produkte)

Synonyme: IFG ELISA Kit, IFI ELISA Kit, IFN-g ELISA Kit, Ifg ELISA Kit, IFNG2 ELISA Kit, IFN-gamma ELISA Kit, IFN-G ELISA Kit, IFNG ELISA Kit, IFNgamma ELISA Kit, TCRalpha ELISA Kit, INF-G ELISA Kit, ifng ELISA Kit, interferon gamma ELISA Kit, interferon, gamma 1-2 ELISA Kit, IFNG ELISA Kit, Ifng ELISA Kit, ifng1-2 ELISA Kit

Hintergrund:

Protein Function: Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.

Background: Interferon-gamma(IFN-gamma) is an inflammatory cytokine that has been implicated in the development of fibrosis in inflamed tissues. The production of IFN-gamma, which is under genetic control, can influence the development of fibrosis in lung allografts. IFN-gamma is also produced by natural killer(NK) cells and most prominently by CD8 cytotoxic T cells, and is vital for the control of microbial pathogens. Interferon gamma is believed to be crucial for host defence against many infections. Genetically determined variability in IFN-gamma and expression might be important for the development of tuberculosis. IFN-gamma activates human macrophage oxidative metabolism and antimicrobial activity. In addition to having antiviral activity, IFN-gamma has important immunoregulatory functions. IFN-gamma plays an important role in the control of neointima proliferation.

Synonyms: Interferon gamma,IFN-gamma,Ifng,

Full Gene Name: Interferon gamma

Cellular Localisation: Secreted.

Gen-ID: 15978

UniProt: P01580

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagie