IGFBPI ELISA Kit

Produktnummer ABIN411288

Produktdetails IGFBPI ELISA Kit

Target: IGFBPI (Insulin-Like Growth Factor Binding Protein 1 (IGFBPI))

Bindungsspezifität: AA 26-259

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.2-2000 pg/mL

Untere Nachweisgrenze: 31.2 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IGFBP-1

Marke: PicoKine™

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Urine

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NSO
Immunogen sequence: A26-N259

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <1pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: A26-N259

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Sequence similarities: Contains 1 IGFBP N-terminal domain.

Plattentyp: Pre-coated

Protokoll: human IGFBP-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IGFBP-1 has been precoated onto 96-well plates. Standards(NSO, A26-N259) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGFBP-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IGFBP-1 amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human IGFBP-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma (heparin, EDTA) or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human IGFBP-1 standard solution and each sample be measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 145, Standard deviation: 6.09, CV(%): 4.2
• Sample 2: n=16, Mean(pg/ml): 736, Standard deviation: 37.54, CV(%): 5.1
• Sample 3: n=16, Mean(pg/ml): 1243, Standard deviation: 70.85, CV(%): 5.7,
• Sample 1: n=24, Mean(pg/ml): 173, Standard deviation: 10.21, CV(%): 5.9
• Sample 2: n=24, Mean(pg/ml): 667, Standard deviation: 42.02, CV(%): 6.3
• Sample 3: n=24, Mean(pg/ml): 1360, Standard deviation: 102, CV(%): 7.5

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Referenzen für IGFBPI ELISA Kit (ABIN411288)

Zhang, Zhao, Hu, Liu, Chen, Chen, Du: "Distinct post-transcriptional regulation of Igfbp1 gene by hypoxia in lowland mouse and Qinghai-Tibet plateau root vole Microtus oeconomus." in: Molecular and cellular endocrinology, Vol. 376, Issue 1-2, pp. 33-42, (2013) (PubMed).

Wang, Wang, Liang, Liu, Shi, Bai, Lin, Magaye, Zhao: "Expression and clinical significance of IGF-1, IGFBP-3, and IGFBP-7 in serum and lung cancer tissues from patients with non-small cell lung cancer." in: OncoTargets and therapy, Vol. 6, pp. 1437-44, (2013) (PubMed).

Details zu IGFBPI

Target: IGFBPI (Insulin-Like Growth Factor Binding Protein 1 (IGFBPI))

Andere Bezeichnung: IGFBP1 (IGFBPI Produkte)

Synonyme: cb656 ELISA Kit, igfbp1 ELISA Kit, IGFBP-1 ELISA Kit, MGC68538 ELISA Kit, afbp ELISA Kit, ibp1 ELISA Kit, pp12 ELISA Kit, igf-bp25 ELISA Kit, higfbp-1 ELISA Kit, IGFBP1 ELISA Kit, LOC100223208 ELISA Kit, LOC100305121 ELISA Kit, AFBP ELISA Kit, IBP1 ELISA Kit, IGF-BP25 ELISA Kit, PP12 ELISA Kit, hIGFBP-1 ELISA Kit, IGFBA ELISA Kit, insulin like growth factor binding protein 1 ELISA Kit, insulin-like growth factor binding protein 1a ELISA Kit, insulin like growth factor binding protein 1 S homeolog ELISA Kit, insulin like growth factor binding protein 1 L homeolog ELISA Kit, insulin-like growth factor binding protein 1 ELISA Kit, IGFBP1 ELISA Kit, igfbp1a ELISA Kit, igfbp1.S ELISA Kit, igfbp1.L ELISA Kit, igfbp1 ELISA Kit, LOC100305121 ELISA Kit, Igfbp1 ELISA Kit

Hintergrund:

Protein Function: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration. .

Background: IGFBP1 is also known as amniotic fluid binding protein(AFBP), placental protein-12, alpha-pregnancy-associated endometrial globulin, growth hormone independent binding protein, binding protein-28, binding protein-26, and binding protein-25. The low-molecular weight insulin-like growth-factor binding protein(IGF-BP25) is synthesized by human liver, secretory endometrium and decidua, and is also present in human serum. It binds insulin-like growth factors IGF-I and IGF-II with high affinity, and is proposed to act as a paracrine regulator of cell growth. These IGF-binding proteins are expressed at different concentrations in different tissues and are thought to regulate the activity of IGF I and II. The gene is organized in four exons and spans 5.9 kb. The IBP-1 gene is a single copy gene, located on chromosome 7. The standard product used in this kit is recombinant human IGFBP-1, consisting of 234 amino acids with the molecular mass of 25KDa.

Synonyms: Insulin-like growth factor-binding protein 1,IBP-1,IGF-binding protein 1,IGFBP-1,Placental protein 12,PP12,IGFBP1,IBP1,

Full Gene Name: Insulin-like growth factor-binding protein 1

Cellular Localisation: Secreted.

Gen-ID: 3484

UniProt: P08833

Pathways: Myometrial Relaxation and Contraction, ER-Nucleus Signaling, Growth Factor Binding