IL-1 beta ELISA Kit

Produktnummer ABIN411294

Produktdetails IL-1 beta ELISA Kit

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Bindungsspezifität: AA 118-269

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 12.5-800 pg/mL

Untere Nachweisgrenze: 12.5 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse IL-1 beta

Marke: PicoKine™

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytische Methode: Quantitative

Spezifität: Expression system for standard: E.coli,V118-S269

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <1pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: E.coli
Immunogen sequence: V118-S269

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plattentyp: Pre-coated

Protokoll: mouse IL-1 beta ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-1 beta has been precoated onto 96-well plates. Standards (E.coli,V118-S269) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-1 beta is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-1 beta amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 800pg/mL, 400pg/mL, 200pg/mL, 100pg/mL, 50pg/mL, 25pg/mL, 12.5pg/mL mouse IL-1 beta standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernatants, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse IL-1 betastandard solution and each sample is measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 86, Standard deviation: 6.97, CV(%): 8.1
• Sample 2: n=16, Mean(pg/ml): 376, Standard deviation: 19.55, CV(%): 5.2
• Sample 3: n=16, Mean(pg/ml): 587, Standard deviation: 24.07, CV(%): 4.1,
• Sample 1: n=24, Mean(pg/ml): 79, Standard deviation: 7.03, CV(%): 8.9
• Sample 2: n=24, Mean(pg/ml): 346, Standard deviation: 25.6, CV(%): 7.4
• Sample 3: n=24, Mean(pg/ml): 558, Standard deviation: 37.94, CV(%): 6.8

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Referenzen für IL-1 beta ELISA Kit (ABIN411294)

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Details zu IL-1 beta

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Andere Bezeichnung: IL1B (IL1B Produkte)

Synonyme: IL-1 ELISA Kit, IL1-BETA ELISA Kit, IL1F2 ELISA Kit, IL-1BETA ELISA Kit, IL1beta ELISA Kit, il1-b ELISA Kit, zgc:111873 ELISA Kit, IL-1B ELISA Kit, IL-1beta ELISA Kit, Il-1b ELISA Kit, IL1B ELISA Kit, IL-1 beta ELISA Kit, IL-1b ELISA Kit, interleukin 1 beta ELISA Kit, interleukin 1, beta ELISA Kit, IL1B ELISA Kit, il1b ELISA Kit, Il1b ELISA Kit

Hintergrund:

Protein Function: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.

Background: Interleukin-1beta(IL-1beta) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1beta, a prominent microglia-derived cytokine, caused oligodendrocyte death in coculture with astrocytes and microglia, but not in pure culture of oligodendrocytes alone1. It also can cause nuclear export of a specific NCOR corepressor complex, resulting in derepression of a specific subset of nuclear factor-kappa-B(NFKB)-regulated genes2. Furthermore, Microenvironmental IL-1beta and, to a lesser extent, IL-1alpha are required for in vivo angiogenesis and invasiveness of different tumor cells3. Additional, the cooperation of IL-1beta and PDGFB induces contractile-to-synthetic phenotype modulation of human aortic smooth muscle cells in culture4. Moreover, the association with disease may be explained by the biologic properties of IL-1beta, which is an important proinflammatory cytokine and a powerful inhibitor of gastric acid secretion.

Synonyms: Interleukin-1 beta,IL-1 beta,Il1b,

Full Gene Name: Interleukin-1 beta

Cellular Localisation: Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.

Gen-ID: 16176

UniProt: P10749

Pathways: NF-kappaB Signalweg, Interferon-gamma Pathway, TLR Signalweg, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagie, Cancer Immune Checkpoints, Inflammasome