MMP8 ELISA Kit

Produktnummer ABIN411332

Produktdetails MMP8 ELISA Kit

Target: MMP8 (Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8))

Bindungsspezifität: AA 21-467

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 156-10000 pg/mL

Untere Nachweisgrenze: 156 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human MMP-8

Marke: PicoKine™

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Saliva

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NSO
Immunogen sequence: F21-G467

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <10pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: F21-G467

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Sequence similarities: Belongs to the peptidase M10A family.

Tissue Specificity: Neutrophils.

Plattentyp: Pre-coated

Protokoll: human MMP-8 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MMP-8 has been precoated onto 96-well plates. Standards(NSO, F21-G467) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MMP-8 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human MMP-8 amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human MMP-8 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma (heparin) or saliva to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human MMP-8 standard solution and each sample be measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 1275, Standard deviation: 56.1, CV(%): 4.4
• Sample 2: n=16, Mean(pg/ml): 3563, Standard deviation: 185.3, CV(%): 5.2
• Sample 3: n=16, Mean(pg/ml): 6822, Standard deviation: 395.7, CV(%): 5.8,
• Sample 1: n=24, Mean(pg/ml): 1370, Standard deviation: 69.87, CV(%): 5.1
• Sample 2: n=24, Mean(pg/ml): 3728, Standard deviation: 242.32, CV(%): 6.5
• Sample 3: n=24, Mean(pg/ml): 7142, Standard deviation: 521.4, CV(%): 7.3

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Referenzen für MMP8 ELISA Kit (ABIN411332)

Kumar, Jain, Chauhan, Bharate, Koli, Kumar: "Influence of different forms and materials (zirconia or titanium) of abutments in periimplant soft-tissue healing using matrix metalloproteinase-8: A randomized pilot study." in: The Journal of prosthetic dentistry, (2017) (PubMed).

Stio, Martinesi, Treves, Borgioli: "In vitro response of human peripheral blood mononuclear cells to AISI 316L austenitic stainless steel subjected to nitriding and collagen coating treatments." in: Journal of materials science. Materials in medicine, Vol. 26, Issue 2, pp. 100, (2015) (PubMed).

Akbari, Prabhuji, Karthikeyan, Raghunatha, Narayanan: "Analysis of matrix metalloproteinase-8 levels in gingival crevicular fluid and whole mouth fluid among smokers and nonsmokers using enzyme-linked immune-sorbent assay and a novel chair-side test." in: Journal of Indian Society of Periodontology, Vol. 19, Issue 5, pp. 525-30, (2015) (PubMed).

Details zu MMP8

Target: MMP8 (Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8))

Andere Bezeichnung: MMP8 (MMP8 Produkte)

Synonyme: MMP8 ELISA Kit, BB138268 ELISA Kit, CLG1 ELISA Kit, HNC ELISA Kit, MMP-8 ELISA Kit, PMNL-CL ELISA Kit, matrix metallopeptidase 8 ELISA Kit, neutrophil collagenase ELISA Kit, MMP8 ELISA Kit, Mmp8 ELISA Kit, LOC100339790 ELISA Kit

Hintergrund:

Protein Function: Can degrade fibrillar type I, II, and III collagens.

Background: Matrix metalloproteinase 8(MMP8) also called neutrophil collagenase. Neutrophil collagenase, a member of the family of matrix metalloproteinases, is distinct from the collagenase of skin fibroblasts and synovial cells in substrate specificity and immunologic crossreactivity. MMP8, an enzyme that degrades fibrillar collagens imparting strength to the fetal membranes, is expressed by leukocytes and chorionic cytotrophoblast cells. The human neutrophil collagenase(HNC) cDNA clone has been sequenced and shown to encode a 467-residue protein. Neutrophil collagenase has been found to possess 57 % identity with the deduced protein sequence for fibroblast collagenase with 72 % chemical similarity. Certain regions of the molecule, including the putative zinc-binding region, are highly conserved. When compared with the published sequence for fibroblast collagenase, neutrophil collagenase contains four additional sites for glycosylation. The standard product used in this kit is natural, isolating from human MMP-8. The detected MMP-8 includes zymogen and active enzyme.

Synonyms: Neutrophil collagenase,3.4.24.34,Matrix metalloproteinase-8,MMP-8,PMNL collagenase,PMNL-CL,MMP8,CLG1,

Full Gene Name: Neutrophil collagenase

Cellular Localisation: Cytoplasmic granule. Secreted, extracellular space, extracellular matrix . Stored in intracellular granules.

Gen-ID: 4317

UniProt: P22894