P-Selectin ELISA Kit

Produktnummer ABIN411352

Produktdetails P-Selectin ELISA Kit

Target: P-Selectin (SELP) (Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP))

Bindungsspezifität: AA 42-709

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 62.5-4000 pg/mL

Untere Nachweisgrenze: 62.5 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse P-Selectin

Marke: PicoKine™

Proben: Cell Culture Supernatant, Serum, Plasma (citrate)

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NSO
Immunogen sequence: W42-A709

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <5pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: W42-A709

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Sequence similarities: Belongs to the selectin/LECAM family.

Tissue Specificity: Stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.

Plattentyp: Pre-coated

Protokoll: mouse P-Selectin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for P-Selectin has been precoated onto 96-well plates. Standards(NSO, W42-A709) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for P-Selectin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse P-Selectin amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse P-Selectin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(citrate) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse P-Selectin standard solution and each sample be measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 325, Standard deviation: 14, CV(%): 4.3
• Sample 2: n=16, Mean(pg/ml): 874, Standard deviation: 43.7, CV(%): 5
• Sample 3: n=16, Mean(pg/ml): 1886, Standard deviation: 71.7, CV(%): 3.8,
• Sample 1: n=24, Mean(pg/ml): 417, Standard deviation: 27.11, CV(%): 6.5
• Sample 2: n=24, Mean(pg/ml): 1029, Standard deviation: 63.8, CV(%): 6.2
• Sample 3: n=24, Mean(pg/ml): 2020, Standard deviation: 94.94, CV(%): 4.7

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Referenzen für P-Selectin ELISA Kit (ABIN411352)

Li, Ben, Yuan, Liu, Wu, Xu, Zhang: "Recruitment of HIV-1 target cells at topical mucosal sites: a sensitive and early marker for determining the safety of microbicide candidates." in: Emerging microbes & infections, Vol. 2, Issue 7, pp. e42, (2015) (PubMed).

Tun, Yasukawa, Yamada: "Involvement of nitric oxide with activation of Toll-like receptor 4 signaling in mice with dextran sodium sulfate-induced colitis." in: Free radical biology & medicine, Vol. 74, pp. 108-17, (2014) (PubMed).

Zhong, He, Yang, Bao, Zhang, Zhou, Chen, Li, Han, Yang, Sun, Cao, Li, Shi, Jiang, Zhang, Yan: "L-selectin and P-selectin are novel biomarkers of cervicovaginal inflammation for preclinical mucosal safety assessment of anti-HIV-1 microbicide." in: Antimicrobial agents and chemotherapy, Vol. 56, Issue 6, pp. 3121-32, (2012) (PubMed).

Yasukawa, Tokuda, Tun, Utsumi, Yamada: "The detrimental effect of nitric oxide on tissue is associated with inflammatory events in the vascular endothelium and neutrophils in mice with dextran sodium sulfate-induced colitis." in: Free radical research, Vol. 46, Issue 12, pp. 1427-36, (2012) (PubMed).

Details zu P-Selectin

Target: P-Selectin (SELP) (Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP))

Andere Bezeichnung: SELP (SELP Produkte)

Synonyme: PSELECT ELISA Kit, CD62 ELISA Kit, CD62P ELISA Kit, GMP140 ELISA Kit, GRMP ELISA Kit, LECAM3 ELISA Kit, PADGEM ELISA Kit, PSEL ELISA Kit, GMP-140 ELISA Kit, Grmp ELISA Kit, selectin P ELISA Kit, selectin, platelet ELISA Kit, Selp ELISA Kit, SELP ELISA Kit

Hintergrund:

Protein Function: Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1. .

Background: P-selectin, also called GMP-140, CD62, or selectin P, is a 140-kD adhesion molecule, expressed at the surface of activated cells, that mediates the interaction of activated endothelial cells or platelets with leukocytes. It is stored in secretory granules and expressed at the plasma membrane after cell activation. It is known to play an important role in atherosclerosis. The major ligand for P-selectin on leukocytes is P-selectin glycoprotein ligand-1(PSGL-1). The standard product used in this kit is recombinant mouse P-Selectin, excluding intercellular P-Selectin and transmembrane domain. It has 42-709 amino acids sequence with the molecular mass of 99.1KDa. As a result of glycosylation, the molecular mass of 190-200KDa is revealed by SDS-PAGE.

Synonyms: P-selectin,CD62 antigen-like family member P,Granule membrane protein 140,GMP-140,Leukocyte-endothelial cell adhesion molecule 3,LECAM3,Platelet activation dependent granule-external membrane protein,PADGEM,CD62P,Selp,Grmp,

Full Gene Name: P-selectin

Cellular Localisation: Membrane, Single-pass type I membrane protein.

Gen-ID: 20344

UniProt: Q01102