PLAU ELISA Kit

Produktnummer ABIN411365

Produktdetails PLAU ELISA Kit

Target: PLAU (Plasminogen Activator, Urokinase (PLAU))

Bindungsspezifität: AA 1-431

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 62.5-4000 pg/mL

Untere Nachweisgrenze: 62.5 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human uPA/PLAU

Marke: PicoKine™

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NSO
Immunogen sequence: M1-L431

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <5pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: M1-L431

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Sequence similarities: Belongs to the peptidase S1 family.

Tissue Specificity: Expressed in the prostate gland and prostate cancers. .

Plattentyp: Pre-coated

Protokoll: human uPA ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for uPA has been precoated onto 96-well plates. Standards(NSO, M1-L431) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for uPA is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human uPA amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human uPA standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human uPA standard solution and each sample be measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 126, Standard deviation: 5.92, CV(%): 4.7
• Sample 2: n=16, Mean(pg/ml): 1895, Standard deviation: 64.43, CV(%): 3.4
• Sample 3: n=16, Mean(pg/ml): 2619, Standard deviation: 138.8, CV(%): 5.3,
• Sample 1: n=24, Mean(pg/ml): 250, Standard deviation: 13.5, CV(%): 5.4
• Sample 2: n=24, Mean(pg/ml): 1568, Standard deviation: 95.65, CV(%): 6.1
• Sample 3: n=24, Mean(pg/ml): 2755, Standard deviation: 187.3, CV(%): 6.8

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Referenzen für PLAU ELISA Kit (ABIN411365)

Bi, Zeng, Zhao, Wei, Yu, Wang, Yu, Cao, Shan, Wei: "miR-181a Induces Macrophage Polarized to M2 Phenotype and Promotes M2 Macrophage-mediated Tumor Cell Metastasis by Targeting KLF6 and C/EBPα." in: Molecular therapy. Nucleic acids, Vol. 5, Issue 9, pp. e368, (2016) (PubMed).

Bronckaers, Hilkens, Fanton, Struys, Gervois, Politis, Martens, Lambrichts: "Angiogenic properties of human dental pulp stem cells." in: PLoS ONE, Vol. 8, Issue 8, pp. e71104, (2013) (PubMed).

Kim, Lee, Choi, Yoo, Yang: "Implication of MMP-9 and urokinase plasminogen activator (uPA) in the activation of pro-matrix metalloproteinase (MMP)-13." in: Rheumatology international, Vol. 32, Issue 10, pp. 3069-75, (2012) (PubMed).

Jiang, Zhang, Zhou, Zhang, Li, Zhang, Zhang, Qiao, Kong, Ma, Chen: "Relationships of uPA and VEGF expression in esophageal cancer and microvascular density with tumorous invasion and metastasis." in: Asian Pacific journal of cancer prevention : APJCP, Vol. 13, Issue 7, pp. 3379-83, (2012) (PubMed).

Wang, Li, Han, Li, Mi, Sun, Song, Han, Zhao, Wang: "Integrin ?3 and its ligand regulate the expression of uPA through p38 MAPK in breast cancer." in: APMIS : acta pathologica, microbiologica, et immunologica Scandinavica, Vol. 118, Issue 12, pp. 909-17, (2010) (PubMed).

Yang, Shi, Li, Yi: "Effects of shRNA targeting maspin on invasion of gastric carcinoma SGC7901 cell line." in: Oncology reports, Vol. 25, Issue 1, pp. 259-65, (2010) (PubMed).

Details zu PLAU

Target: PLAU (Plasminogen Activator, Urokinase (PLAU))

Andere Bezeichnung: PLAU (PLAU Produkte)

Synonyme: PLAU ELISA Kit, u-PA ELISA Kit, ATF ELISA Kit, BDPLT5 ELISA Kit, QPD ELISA Kit, UPA ELISA Kit, URK ELISA Kit, uPA ELISA Kit, UPAM ELISA Kit, plasminogen activator, urokinase ELISA Kit, urokinase-type plasminogen activator ELISA Kit, PLAU ELISA Kit, CpipJ_CPIJ002131 ELISA Kit, CpipJ_CPIJ006543 ELISA Kit, CpipJ_CPIJ013396 ELISA Kit, CpipJ_CPIJ013623 ELISA Kit, Plau ELISA Kit

Hintergrund:

Protein Function: Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin.

Background: Plasminogen activator,urokinase(PLAU, uPA) converts plasminogen to plasmin. Plasmin is involved in processing of amyloid precursor protein and degrades secreted and aggregated amyloid-beta, a hallmark of Alzheimer disease(AD). Urokinase has a molecular mass of about 54 kD and is composed of 2 disulfide-linked chains, A and B, of molecular masses 18 kD and 33 kD, respectively. It localized on 10q24. uPA facilitates cell migration by localizing proteolisys on the cell surface and by inducing intracellular signalling pathways. In human vascular smooth muscle cell(VSMC), uPA stimulates migration via the uPA receptor(uPAR) signalling complex containing TYK2 and phosphatidylinositol 3-kinase(PI3-K).

Synonyms: Urokinase-type plasminogen activator,U-plasminogen activator,uPA,3.4.21.73,Urokinase-type plasminogen activator long chain A,Urokinase-type plasminogen activator short chain A,Urokinase-type plasminogen activator chain B,PLAU,

Full Gene Name: Urokinase-type plasminogen activator

Cellular Localisation: Secreted.

Gen-ID: 5328

UniProt: P00749

Pathways: Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagie, Smooth Muscle Cell Migration