Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

PLAUR ELISA Kit

PLAUR Reaktivität: Human AA 23-303 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum, Urine
Produktnummer ABIN411366
  • Target Alle PLAUR ELISA Kits anzeigen
    PLAUR (Plasminogen Activator, Urokinase Receptor (PLAUR))
    Bindungsspezifität
    AA 23-303
    Reaktivität
    • 8
    • 7
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    62.5-4000 pg/mL
    Untere Nachweisgrenze
    62.5 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human uPAR
    Marke
    PicoKine™
    Proben
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Urine
    Analytische Methode
    Quantitative
    Spezifität
    Expression system for standard: NSO,L23-R303
    Kreuzreaktivität (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivität
    <4pg/mL
    Benötigtes Material
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: NSO
    Immunogen sequence: L23-R303
    Featured
    Zu unserem meistverkauften PLAUR ELISA Kit
    Top Product
    Discover our top product PLAUR ELISA Kit
  • Applikationshinweise
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Kommentare

    Sequence similarities: Contains 3 UPAR/Ly6 domains.

    Tissue Specificity: Expressed in neurons of the rolandic area of the brain (at protein level). Expressed in the brain.

    Plattentyp
    Pre-coated
    Protokoll
    human uPAR ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for uPAR has been precoated onto 96-well plates. Standards (NSO,L23-R303) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for uPAR is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human uPAR amount of sample captured in plate.
    Testdurchführung

    Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human uPAR standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or urine to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human uPAR standard solution and each sample is measured in duplicate.

    Testpräzision
    • Sample 1: n=16, Mean(pg/ml): 240, Standard deviation: 8.16, CV(%): 3.4
    • Sample 2: n=16, Mean(pg/ml): 1437, Standard deviation: 73.3, CV(%): 5.1
    • Sample 3: n=16, Mean(pg/ml): 2682, Standard deviation: 120.7, CV(%): 4.5,
    • Sample 1: n=24, Mean(pg/ml): 365, Standard deviation: 17.52, CV(%): 4.8
    • Sample 2: n=24, Mean(pg/ml): 1624, Standard deviation: 105.6, CV(%): 6.5
    • Sample 3: n=24, Mean(pg/ml): 3163, Standard deviation: 186.6, CV(%): 5.9
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    Avoid multiple freeze-thaw cycles.
    Lagerung
    -20 °C,4 °C
    Informationen zur Lagerung
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Haltbarkeit
    12 months
  • Target Alle PLAUR ELISA Kits anzeigen
    PLAUR (Plasminogen Activator, Urokinase Receptor (PLAUR))
    Andere Bezeichnung
    PLAUR (PLAUR Produkte)
    Synonyme
    Cd87 ELISA Kit, u-PAR ELISA Kit, uPAR ELISA Kit, CD87 ELISA Kit, U-PAR ELISA Kit, UPAR ELISA Kit, URKR ELISA Kit, Par ELISA Kit, Plaur3 ELISA Kit, uPAR-2 ELISA Kit, uPAR-3 ELISA Kit, PLAUR ELISA Kit, plasminogen activator, urokinase receptor ELISA Kit, Plaur ELISA Kit, PLAUR ELISA Kit
    Hintergrund

    Protein Function: Acts as a receptor for urokinase plasminogen activator. Plays a role in localizing and promoting plasmin formation. Mediates the proteolysis-independent signal transduction activation effects of U-PA. It is subject to negative-feedback regulation by U-PA which cleaves it into an inactive form.

    Background: The urokinase-type plasminogen activator receptor(uPAR) is a key molecule in the regulation of cell-surface plasminogen activation and, as such, plays an important role in many normal as well as pathological processes. The cDNA for Mo3, an activation antigen expressed by human monocytes and myelomonocytic cell lines after stimulation by a variety of agents. Mo3 expression in vivo is associated predominantly with macrophages in inflammatory sites. It is a highly glycosylated protein of about 50 kD in monocytes where it is anchored to the plasma membrane by glycosyl-phosphatidylinositol linkage. The complete coding sequence of the cDNA has been found to encode 335 amino acids including a predicted signal peptide of 22 residues and a hydrophobic C-terminal portion. Mo3 is identical to the human receptor for the urokinase plasminogen activator. UPAR is a useful prognostic marker for biologically aggressive forms of endometrial cancer. PLAUR is located at chromosome 19q13.1-q13.2. The standard product used in this kit is recombinant human uPAR, consisting of 287 amino acids with the molecular mass of 31KDa.

    Synonyms: Urokinase plasminogen activator surface receptor,U-PAR,uPAR,Monocyte activation antigen Mo3,CD87,PLAUR,MO3, UPAR,

    Full Gene Name: Urokinase plasminogen activator surface receptor

    Cellular Localisation: Cell membrane . Cell projection, invadopodium membrane . Colocalized with FAP (seprase) preferentially at the cell surface of invadopodia membrane in a cytoskeleton-, integrin- and vitronectin-dependent manner..
    Gen-ID
    5329
    UniProt
    Q03405
    Pathways
    Inositol Metabolic Process
Sie sind hier:
Kundenservice