VEGF ELISA Kit

Produktnummer ABIN411369

Produktdetails VEGF ELISA Kit

Target: VEGF (Vascular Endothelial Growth Factor (VEGF))

Bindungsspezifität: AA 27-191

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.2-2000 pg/mL

Untere Nachweisgrenze: 31.2 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human VEGF

Marke: PicoKine™

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)

Analytische Methode: Quantitative

Spezifität: Expression system for standard: sf21,A27-R191

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <1pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: sf21
Immunogen sequence: A27-R191

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Sequence similarities: Belongs to the PDGF/VEGF growth factor family.

Tissue Specificity: Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed. A higher level expression seen in pituitary tumors as compared to the pituitary gland. .

Plattentyp: Pre-coated

Protokoll: human VEGF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for VEGF has been precoated onto 96-well plates. Standards (sf21,A27-R191) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for VEGF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human VEGF amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human VEGF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human VEGF standard solution and each sample is measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 146, Standard deviation: 6.57, CV(%): 4.5
• Sample 2: n=16, Mean(pg/ml): 650, Standard deviation: 24.7, CV(%): 3.8
• Sample 3: n=16, Mean(pg/ml): 1173, Standard deviation: 63.34, CV(%): 5.4,
• Sample 1: n=24, Mean(pg/ml): 242, Standard deviation: 12.1, CV(%): 5
• Sample 2: n=24, Mean(pg/ml): 567, Standard deviation: 27.78, CV(%): 4.9
• Sample 3: n=24, Mean(pg/ml): 1084, Standard deviation: 70.46, CV(%): 6.5

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Referenzen für VEGF ELISA Kit (ABIN411369)

Lin, Fang, Su, Li, Wang, Liao, Hu, Ye, Fang, Luo, Lin, Pan, Wang, Zhang: "DHX32 Promotes Angiogenesis in Colorectal Cancer Through Augmenting β-catenin Signaling to Induce Expression of VEGFA." in: EBioMedicine, Vol. 18, pp. 62-72, (2018) (PubMed).

A, Li, Zhao: "The expression and underlying angiogenesis effect of DPC4 and VEGF on the progression of cervical carcinoma." in: Oncology letters, Vol. 15, Issue 2, pp. 2534-2540, (2018) (PubMed).

Hu, Li, Liu, Xu, Lai, Luo, Hu, Yang: "Histone demethylase KDM4D promotes gastrointestinal stromal tumor progression through HIF1β/VEGFA signalling." in: Molecular cancer, Vol. 17, Issue 1, pp. 107, (2018) (PubMed).

Li, Wu, Chen, Qiu: "Elevated expression levels of serum insulin-like growth factor-1, tumor necrosis factor-α and vascular endothelial growth factor 165 might exacerbate type 2 diabetic nephropathy." in: Journal of diabetes investigation, Vol. 8, Issue 1, pp. 108-114, (2017) (PubMed).

Wang, Cao, Liu, Du, Wang, Chen, Liu, Wu: "5-Aminolaevulinic Acid-Based Photodynamic Therapy Restrains Pathological Hyperplasia of Fibroblasts." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 23, pp. 46-56, (2017) (PubMed).

Arora, Kotwal, Dogra, Doda: "Growth Factor Variation in Two Types of Autologous Platelet Biomaterials: PRP Versus PRF." in: Indian journal of hematology & blood transfusion : an official journal of Indian Society of Hematology and Blood Transfusion, Vol. 33, Issue 2, pp. 288-292, (2017) (PubMed).

Ma, Li, Li, Zhu, Li, Tian: "Long non-coding RNA HOTAIR enhances angiogenesis by induction of VEGFA expression in glioma cells and transmission to endothelial cells via glioma cell derived-extracellular vesicles." in: American journal of translational research, Vol. 9, Issue 11, pp. 5012-5021, (2017) (PubMed).

Fu, Chen, Wang, An, Tao, Zhou, Huang, Chen, Li, You, Guo, Wu: "Wogonin inhibits multiple myeloma-stimulated angiogenesis via c-Myc/VHL/HIF-1α signaling axis." in: Oncotarget, Vol. 7, Issue 5, pp. 5715-27, (2016) (PubMed).

Bozduman, Ersoy Evans, Karahan, Hayran, Akbiyik, Lay: "Genetic Risk Factors for Psoriasis in Turkish Population: -1540 C/A, -1512 Ins18, and +405 C/G Polymorphisms within the Vascular Endothelial Growth Factor Gene." in: Annals of dermatology, Vol. 28, Issue 1, pp. 30-9, (2016) (PubMed).

Fan, Zhang, Xu, Ke, Ding, Wang, Liu, Ning, Deng, He: "Serum level of vascular endothelial growth factor decreased in chronic ketamine abusers." in: Drug and alcohol dependence, Vol. 152, pp. 57-61, (2015) (PubMed).

Das, Jargar, Saha, Yendigeri, Singh: "?-tocopherol supplementation prevents lead acetate and hypoxia-induced hepatic dysfunction." in: Indian journal of pharmacology, Vol. 47, Issue 3, pp. 285-91, (2015) (PubMed).

Fu, Chen, Cheng, Wang: "Hypoxia-inducible factor-1? protects cervical carcinoma cells from apoptosis induced by radiation via modulation of vascular endothelial growth factor and p53 under hypoxia." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 21, pp. 318-25, (2015) (PubMed).

Zhang, Chen, Fan, Wang, Zhu: "Overexpression of VEGF183 promotes murine breast cancer cell proliferation in vitro and induces dilated intratumoral microvessels." in: Tumour biology, Vol. 36, Issue 5, pp. 3871-80, (2015) (PubMed).

Zhang, Fan, Wu, Mu, Wang, Zhu et al.: "[Corrigendum] Overexpression of the chimeric plasmin-resistant VEGF165/VEGF183 (132-158) protein in murine breast cancer induces distinct vascular patterning adjacent to tumors and retarded tumor ..." in: Molecular medicine reports, Vol. 11, Issue 5, pp. 4010, (2015) (PubMed).

Li, Wang, Zhao, Yang, Li, Zhao, Yu, Wang, Meng, Liu, Zhu, Gao, Jia, Yang: "Nuclear factor of activated T cells 5 maintained by Hotair suppression of miR-568 upregulates S100 calcium binding protein A4 to promote breast cancer metastasis." in: Breast cancer research : BCR, Vol. 16, Issue 5, pp. 454, (2015) (PubMed).

Shi, Liu, Zhang, Liu, Lin, Tang: "Epigallocatechin-3-gallate inhibits nicotine-induced migration and invasion by the suppression of angiogenesis and epithelial-mesenchymal transition in non-small cell lung cancer cells." in: Oncology reports, Vol. 33, Issue 6, pp. 2972-80, (2015) (PubMed).

Guo, Sun, Xu, Zhao, Peng, Wang: "Human umbilical cord mesenchymal stem cells promote peripheral nerve repair via paracrine mechanisms." in: Neural regeneration research, Vol. 10, Issue 4, pp. 651-8, (2015) (PubMed).

Sancakdar, Guven, Uysal, Deveci, Gültürk: "Important of Angiopoietic System in Evaluation of Endothelial Damage in Children with Crimean-Congo Hemorrhagic Fever." in: The Pediatric infectious disease journal, Vol. 34, Issue 8, pp. e200-5, (2015) (PubMed).

Xu, Ge, Han, Yang, Su, Zhao, Zhao, Yang, Yang: "High-mobility group box 1 expression and lymph node metastasis in intrahepatic cholangiocarcinoma." in: World journal of gastroenterology : WJG, Vol. 21, Issue 11, pp. 3256-65, (2015) (PubMed).

Chen, Xu, Li, Du, Chen, Liu, Peng, Wang, Song: "Epigallocatechin-3-gallate attenuates lipopolysaccharide-induced mastitis in rats via suppressing MAPK mediated inflammatory responses and oxidative stress." in: International immunopharmacology, Vol. 26, Issue 1, pp. 147-52, (2015) (PubMed).

Details zu VEGF

Target: VEGF (Vascular Endothelial Growth Factor (VEGF))

Abstract: VEGF Produkte

Synonyme: MVCD1 ELISA Kit, VEGF ELISA Kit, VPF ELISA Kit, Vegf ELISA Kit, Vegf120 ELISA Kit, Vegf164 ELISA Kit, Vegf188 ELISA Kit, Vpf ELISA Kit, VEGF-A ELISA Kit, VEGF164 ELISA Kit, eVEGF120 ELISA Kit, eVEGF164 ELISA Kit, vegf-a ELISA Kit, vegf ELISA Kit, vascular endothelial growth factor A ELISA Kit, vascular endothelial growth factor A L homeolog ELISA Kit, vascular endothelial growth factor ELISA Kit, vascular endothelial growth factor precursor ELISA Kit, VEGFA ELISA Kit, Vegfa ELISA Kit, vegfa.L ELISA Kit, VEGF ELISA Kit, vegf ELISA Kit

Hintergrund:

Protein Function: Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. .

Background: Vascular permeability factor/vascular endothelial growth factor(VPF/VEGF), a potent cytokine expressed by most malignant tumors, has critical roles in vasculogenesis and both physiological and pathological angiogenesis.VEGF produced by tumor cells potently stimulates endothelial cell proliferation and angiogenesis and plays a key role in the pathophysiology of several neoplasias. VEGF may also play a pivotal role in mediating the development and progression of diabetic retinopathy.VEGF, a major regulator of angiogenesis, binds to two receptor tyrosine kinases, KDR/Flk-1 and Flt-1. The VEGF gene is mapped by fluorescence in situ hybridization to chromosome 6p12.

Synonyms: Vascular endothelial growth factor A,VEGF-A,Vascular permeability factor,VPF,VEGFA,VEGF,

Full Gene Name: Vascular endothelial growth factor A

Cellular Localisation: Secreted . VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.

Gen-ID: 7422

UniProt: P15692