VEGF ELISA Kit

Produktnummer ABIN411371

Produktdetails VEGF ELISA Kit

Target: VEGF (Vascular Endothelial Growth Factor (VEGF))

Bindungsspezifität: AA 27-190

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.6-1000 pg/mL

Untere Nachweisgrenze: 15.6 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse VEGF

Marke: PicoKine™

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)

Analytische Methode: Quantitative

Spezifität: Expression system for standard: sf21,A27-R190

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <2pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: sf21
Immunogen sequence: A27-R190

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Sequence similarities: Belongs to the PDGF/VEGF growth factor family.

Tissue Specificity: In developing embryos, expressed mainly in the choroid plexus, paraventricular neuroepithelium, placenta and kidney glomeruli. Also found in bronchial epithelium, adrenal gland and in seminiferous tubules of testis. High expression of VEGF continues in kidney glomeruli and choroid plexus in adults.

Plattentyp: Pre-coated

Protokoll: mouse VEGF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for VEGF has been precoated onto 96-well plates. Standards (sf21,A27-R190) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for VEGF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse VEGF amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL mouse VEGF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse VEGF standard solution and each sample is measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 126, Standard deviation: 4.41, CV(%): 3.5
• Sample 2: n=16, Mean(pg/ml): 341, Standard deviation: 13.64, CV(%): 4
• Sample 3: n=16, Mean(pg/ml): 580, Standard deviation: 28.42, CV(%): 4.9,
• Sample 1: n=24, Mean(pg/ml): 212, Standard deviation: 10.6, CV(%): 5
• Sample 2: n=24, Mean(pg/ml): 475, Standard deviation: 30.4, CV(%): 6.4
• Sample 3: n=24, Mean(pg/ml): 624, Standard deviation: 46.8, CV(%): 7.5

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Referenzen für VEGF ELISA Kit (ABIN411371)

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Li, Ding, Ling, Wu, Meng, Li, Zhang: "Metformin attenuates motility, contraction, and fibrogenic response of hepatic stellate cells in vivo and in vitro by activating AMP-activated protein kinase." in: World journal of gastroenterology, Vol. 24, Issue 7, pp. 819-832, (2018) (PubMed).

Zou, Wang, Liu, Bai, Wang, Liu, Zhang, Xiao, Wang: "Nucleolin mediated pro-angiogenic role of Hydroxysafflor Yellow A in ischaemic cardiac dysfunction: Post-transcriptional regulation of VEGF-A and MMP-9." in: Journal of cellular and molecular medicine, Vol. 22, Issue 5, pp. 2692-2705, (2018) (PubMed).

Cheng, Chen, Su, Su, Hou: "A novel dressing seeded with embryonic artery CD133+ cells and loaded with the Sirt1 agonist SRT1720 accelerates the healing of diabetic ischemic ulcers." in: Experimental and therapeutic medicine, Vol. 15, Issue 6, pp. 5243-5250, (2018) (PubMed).

Wang, Liu, Chen, Chen: "Physicochemical characterization of the oolong tea polysaccharides with high molecular weight and their synergistic effects in combination with polyphenols on hepatocellular carcinoma." in: Biomedicine & pharmacotherapy, Vol. 90, pp. 160-170, (2017) (PubMed).

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Li, Wang, Wang, Zhang, Wu, Yang, Ji, Li, Wang: "Occludin downregulation in high glucose is regulated by SSTR2 via the VEGF/NRP1/Akt signaling pathway in RF/6A cells." in: Experimental and therapeutic medicine, Vol. 14, Issue 2, pp. 1732-1738, (2017) (PubMed).

Sun, Chen, Zhang, Shen, Wang, Wang, Liu, Liu, Liu, Liu, Jin: "β2-Adrenergic Receptor-Mediated HIF-1α Upregulation Mediates Blood Brain Barrier Damage in Acute Cerebral Ischemia." in: Frontiers in molecular neuroscience, Vol. 10, pp. 257, (2017) (PubMed).

Abdel-Gawad, Hassan, Awwad: "Efficiency of calcium phosphate composite nanoparticles in targeting Ehrlich carcinoma cells transplanted in mice." in: Journal of advanced research, Vol. 7, Issue 1, pp. 143-54, (2016) (PubMed).

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Details zu VEGF

Target: VEGF (Vascular Endothelial Growth Factor (VEGF))

Abstract: VEGF Produkte

Synonyme: MVCD1 ELISA Kit, VEGF ELISA Kit, VPF ELISA Kit, Vegf ELISA Kit, Vegf120 ELISA Kit, Vegf164 ELISA Kit, Vegf188 ELISA Kit, Vpf ELISA Kit, VEGF-A ELISA Kit, VEGF164 ELISA Kit, eVEGF120 ELISA Kit, eVEGF164 ELISA Kit, vegf-a ELISA Kit, vegf ELISA Kit, vascular endothelial growth factor A ELISA Kit, vascular endothelial growth factor A L homeolog ELISA Kit, vascular endothelial growth factor ELISA Kit, vascular endothelial growth factor precursor ELISA Kit, VEGFA ELISA Kit, Vegfa ELISA Kit, vegfa.L ELISA Kit, VEGF ELISA Kit, vegf ELISA Kit

Hintergrund:

Protein Function: Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. May play a role in increasing vascular permeability during lactation, when increased transport of molecules from the blood is required for efficient milk protein synthesis (By similarity). .

Background: Vascular permeability factor/vascular endothelial growth factor(VPF/VEGF), a potent cytokine expressed by most malignant tumors, has critical roles in vasculogenesis and both physiological and pathological angiogenesis. VEGF produced by tumor cells potently stimulates endothelial cell proliferation and angiogenesis and plays a key role in the pathophysiology of several neoplasias. VEGF may also play a pivotal role in mediating the development and progression of diabetic retinopathy. VEGF, a major regulator of angiogenesis, binds to two receptor tyrosine kinases, KDR/Flk-1 and Flt-1.The VEGF gene is mapped by fluorescence in situ hybridization to chromosome 6p12. The standard product used in this kit is recombinant mouse VEGF164, a 42KDa dimer.

Synonyms: Vascular endothelial growth factor A,VEGF-A,Vascular permeability factor,VPF,Vegfa,Vegf,

Full Gene Name: Vascular endothelial growth factor A

Cellular Localisation: Isoform VEGF-1: Secreted.

Gen-ID: 22339

UniProt: Q00731