ADIPOQ ELISA Kit

Produktnummer ABIN411384

Produktdetails ADIPOQ ELISA Kit

Target: ADIPOQ (Adiponectin (ADIPOQ))

Bindungsspezifität: AA 19-244

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.56-100 ng/mL

Untere Nachweisgrenze: 1.56 ng/mL

Applikation: ELISA

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Adiponectin

Marke: PicoKine™

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Tissue Homogenate, Urine

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NSO
Immunogen sequence: E19-N244

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <60pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: E19-N244

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plattentyp: Pre-coated

Protokoll: human Adiponectin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Adiponectin has been precoated onto 96-well plates. Standards(NSO, E19-N244) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Adiponectin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Adiponectin amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 100 ng/mL, 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL human Adiponectin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA), tissue homogenates or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Adiponectin standard solution and each sample be measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(ng/ml): 13.8, Standard deviation: 0.39, CV(%): 2.8
• Sample 2: n=16, Mean(ng/ml): 37.2, Standard deviation: 1.34, CV(%): 3.6
• Sample 3: n=16, Mean(ng/ml): 67.3, Standard deviation: 3.03, CV(%): 4.5,
• Sample 1: n=24, Mean(ng/ml): 13.1, Standard deviation: 0.77, CV(%): 5.9
• Sample 2: n=24, Mean(ng/ml): 36.8, Standard deviation: 2.50, CV(%): 6.8
• Sample 3: n=24, Mean(ng/ml): 65.7, Standard deviation: 4.66, CV(%): 7.1

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Referenzen für ADIPOQ ELISA Kit (ABIN411384)

Buturak, Değirmencioğlu, Bayrak, Kırış, Karakurt, Demir, Sürgit, Ertürk: "Elective percutaneous coronary intervention leads to significant changes in serum resistin, leptin, and adiponectin levels regardless of periprocedural myocardial injury: an observational study." in: Anatolian journal of cardiology, Vol. 16, Issue 12, pp. 940-946, (2018) (PubMed).

Altay, Seçil, Demir, Atik, Akıncı, Özdemir Kutbay, Keskin Temeloğlu, Yıldırım Şimşir, Özışık, Demir, Eren, Tuna, Aytaç, Onay, Akıncı: "Determining residual adipose tissue characteristics with MRI in patients with various subtypes of lipodystrophy." in: Diagnostic and interventional radiology (Ankara, Turkey), Vol. 23, Issue 6, pp. 428-434, (2018) (PubMed).

Küme, Acar, Tuhan, Çatlı, Anık, Gürsoy Çalan, Böber, Abacı: "The Relationship between Serum Zonulin Level and Clinical and Laboratory Parameters of Childhood Obesity." in: Journal of clinical research in pediatric endocrinology, Vol. 9, Issue 1, pp. 31-38, (2017) (PubMed).

Koh, Park: "Responses of inflammatory cytokines following moderate intensity walking exercise in overweight or obese individuals." in: Journal of exercise rehabilitation, Vol. 13, Issue 4, pp. 472-476, (2017) (PubMed).

Mohammadi, Paknahad: "Adiponectin Concentration in Gestational Diabetic Women: a Case-Control Study." in: Clinical nutrition research, Vol. 6, Issue 4, pp. 267-276, (2017) (PubMed).

Naini, Vahdat, Hedaiati, Shahzeidi, Pezeshki, Nasri et al.: "The effect of vitamin D administration on serum leptin and adiponectin levels in end-stage renal disease patients on hemodialysis with vitamin D deficiency: A placebo-controlled double-blind clinical ..." in: Journal of research in medical sciences : the official journal of Isfahan University of Medical Sciences, Vol. 21, pp. 1, (2016) (PubMed).

Wu, Weng, Lai, Tsai, Liu, Lee, Tsai: "Effect of Lactobacillus plantarum Strain K21 on High-Fat Diet-Fed Obese Mice." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2015, pp. 391767, (2015) (PubMed).

Rasekhi, Karandish, Jalali, Mohammadshahi, Zarei, Saki, Shahbazian: "Phylloquinone supplementation improves glycemic status independent of the effects of adiponectin levels in premonopause women with prediabetes: a double-blind randomized controlled clinical trial." in: Journal of diabetes and metabolic disorders, Vol. 14, Issue 1, pp. 1, (2015) (PubMed).

Liu, Liu, Li, Han, Peng, Shen: "Adiponectin is expressed in the pancreas of high-fat-diet-fed mice and protects pancreatic endothelial function during the development of type 2 diabetes." in: Diabetes & metabolism, Vol. 40, Issue 5, pp. 363-72, (2014) (PubMed).

Hatami Zargaran, Salehi, Heydari, Babajafari: "The Effects of 6 Isocaloric Meals on Body Weight, Lipid Profiles, Leptin, and Adiponectin in Overweight Subjects (BMI > 25)." in: International cardiovascular research journal, Vol. 8, Issue 2, pp. 52-6, (2014) (PubMed).

Liu, Gu, Zhao, An, Yang: "Glypican 4 may be involved in the adipose tissue redistribution in high-fat feeding C57BL/6J mice with peroxisome proliferators-activated receptor ? agonist rosiglitazone treatment." in: Experimental and therapeutic medicine, Vol. 8, Issue 6, pp. 1813-1818, (2014) (PubMed).

Chen, Li, Zhang, Li, Sun, Zhang, Feng, Zhu: "Effects of resveratrol on the amelioration of insulin resistance in KKAy mice." in: Canadian journal of physiology and pharmacology, Vol. 90, Issue 2, pp. 237-42, (2012) (PubMed).

Details zu ADIPOQ

Target: ADIPOQ (Adiponectin (ADIPOQ))

Andere Bezeichnung: ADIPOQ (ADIPOQ Produkte)

Synonyme: ADP ELISA Kit, DCAF9 ELISA Kit, 6720489L24Rik ELISA Kit, 9330209L04Rik ELISA Kit, Adp ELISA Kit, Mamdc1 ELISA Kit, RNSVSP4U ELISA Kit, SVSIV1 ELISA Kit, Svp4 ELISA Kit, ACDC ELISA Kit, ACRP30 ELISA Kit, ADIPQTL1 ELISA Kit, ADPN ELISA Kit, APM-1 ELISA Kit, APM1 ELISA Kit, GBP28 ELISA Kit, apM-1 ELISA Kit, 30kDa ELISA Kit, APN ELISA Kit, Acdc ELISA Kit, Acrp30 ELISA Kit, Ad ELISA Kit, adipo ELISA Kit, apM1 ELISA Kit, ADN ELISA Kit, acrpl ELISA Kit, adipo-b ELISA Kit, adipoql ELISA Kit, zgc:153584 ELISA Kit, adipoq ELISA Kit, WD and tetratricopeptide repeats 1 ELISA Kit, MAM domain containing glycosylphosphatidylinositol anchor 2 ELISA Kit, seminal vesicle secretory protein 4 ELISA Kit, adiponectin, C1Q and collagen domain containing ELISA Kit, adiponectin, C1Q and collagen domain containing, b ELISA Kit, adiponectin, C1Q and collagen domain containing L homeolog ELISA Kit, WDTC1 ELISA Kit, Mdga2 ELISA Kit, Svs4 ELISA Kit, ADIPOQ ELISA Kit, Adipoq ELISA Kit, adipoqb ELISA Kit, adipoq.L ELISA Kit

Hintergrund:

Background: Adiponectin(ADPN) is a hormone secreted by adipocytes that regulates energy homeostasis and glucose and lipid metabolism. Adiponectin is a new member of the family of soluble defense collagens, in hematopoiesis and immune responses. It is an important negative regulator in hematopoiesis and immune systems and raise the possibility that it may be involved in ending inflammatory responses through its inhibitory functions. Adiponectin is mapped to 3q27 and can protect the organism from systemic inflammation by promoting the clearance of early apoptotic cells by macrophages through a receptor-dependent pathway involving calreticulin. The standard product used in this kit is the product of gene recombination, consisting of 226(19-244) amino acids with the molecular mass of 36KDa after glycosylation.

Synonyms: Adiponectin ,Adiponectin, C1Q and collagen domain containing ,cDNA FLJ78108, highly similar to Homo sapiens adiponectin, C1Q and collagen domain containing (ADIPOQ), mRNA ,ADIPOQ ,hCG_1784052 ,

Full Gene Name: adiponectin, C1Q and collagen domain containing

Gen-ID: 9370

UniProt: A8K660