Lipocalin 2 ELISA Kit

Produktnummer ABIN411403

Produktdetails Lipocalin 2 ELISA Kit

Target: Lipocalin 2 (LCN2)

Bindungsspezifität: AA 21-198

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 78-5000 pg/mL

Untere Nachweisgrenze: 78 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat Lipocalin-2/NGAL

Marke: PicoKine™

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Urine

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NSO
Immunogen sequence: Q21-N198

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <10pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: Q21-N198

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plattentyp: Pre-coated

Protokoll: rat NGAL ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for NGAL has been precoated onto 96-well plates. Standards(NSO, Q21-N198) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for NGAL is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat NGAL amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL, 78pg/mL rat NGAL standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum, plasma(heparin) or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat NGAL standard solution and each sample be measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 422, Standard deviation: 18.99, CV(%): 4.5
• Sample 2: n=16, Mean(pg/ml): 1667, Standard deviation: 86.7, CV(%): 5.2
• Sample 3: n=16, Mean(pg/ml): 2922, Standard deviation: 163.6, CV(%): 5.6,
• Sample 1: n=24, Mean(pg/ml): 638, Standard deviation: 38.92, CV(%): 6.1
• Sample 2: n=24, Mean(pg/ml): 1923, Standard deviation: 130.8, CV(%): 6.8
• Sample 3: n=24, Mean(pg/ml): 3125, Standard deviation: 231, CV(%): 7.4

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Referenzen für Lipocalin 2 ELISA Kit (ABIN411403)

Özlülerden, Toktaş, Aybek, Küçükatay, Şen Türk, Zumrutbas: "The renoprotective effects of mannitol and udenafil in renal ischemia-reperfusion injury model." in: Investigative and clinical urology, Vol. 58, Issue 4, pp. 289-295, (2018) (PubMed).

Erkılıç, Kesimci, Alaybeyoğlu, Kılınç, Tural, Yazgan, Gümüş, Sepici Dinçel, Dumlu, Kanbak: "Does remifentanil attenuate renal ischemia-reperfusion injury better than dexmedetomidine in rat kidney?" in: Drug design, development and therapy, Vol. 11, pp. 677-683, (2017) (PubMed).

Wang, Liu, He, Lv, Du: "Esculin improves dyslipidemia, inflammation and renal damage in streptozotocin-induced diabetic rats." in: BMC complementary and alternative medicine, Vol. 15, pp. 402, (2016) (PubMed).

Ozbilgin, Ozkardesler, Akan, Boztas, Ozbilgin, Ergur, Derici, Guneli, Meseri: "Renal Ischemia/Reperfusion Injury in Diabetic Rats: The Role of Local Ischemic Preconditioning." in: BioMed research international, Vol. 2016, pp. 8580475, (2016) (PubMed).

Li, Jia, Ji, Gong, Wang, Zhang, Wang, Zang: "The neutrophil elastase inhibitor, sivelestat, attenuates sepsis-related kidney injury in rats." in: International journal of molecular medicine, Vol. 38, Issue 3, pp. 767-75, (2016) (PubMed).

Guo, Fang, You, Jin, Wang, Hu, Han: "Effects of hydrogen-rich saline on early acute kidney injury in severely burned rats by suppressing oxidative stress induced apoptosis and inflammation." in: Journal of translational medicine, Vol. 13, pp. 183, (2015) (PubMed).

Guo, Zhou, Huang, You, Fang, Wu, Wang, Han: "Astaxanthin attenuates early acute kidney injury following severe burns in rats by ameliorating oxidative stress and mitochondrial-related apoptosis." in: Marine drugs, Vol. 13, Issue 4, pp. 2105-23, (2015) (PubMed).

Liu, Dong, Sun, Liu: "A novel fluid resuscitation protocol: provide more protection on acute kidney injury during septic shock in rats." in: International journal of clinical and experimental medicine, Vol. 7, Issue 4, pp. 919-26, (2014) (PubMed).

Koca, Olguner, Ergür, Altekin, Ta?dö?en, Duru, Girgin, Gündüz, Cilaker M?c?l?, Güzelda?, Akku?: "The effects of dexmedetomidine on secondary acute lung and kidney injuries in the rat model of intra-abdominal sepsis." in: TheScientificWorldJournal, Vol. 2013, pp. 292687, (2013) (PubMed).

Olguner, Koca, Altekin, Ergür, Duru, Girgin, Ta?dö?en, Gündüz, Güzelda?, Akku?, Micili: "Ischemic preconditioning attenuates lipid peroxidation and apoptosis in the cecal ligation and puncture model of sepsis." in: Experimental and therapeutic medicine, Vol. 5, Issue 6, pp. 1581-1588, (2013) (PubMed).

Sun, Meng, Jiang, Liu, Lei, Su, Duan, Wu, Xia, Xia: "Protective effect of ginsenoside Rb1 against intestinal ischemia-reperfusion induced acute renal injury in mice." in: PLoS ONE, Vol. 8, Issue 12, pp. e80859, (2013) (PubMed).

Hur, Garip, Camyar, Ilgun, Ozisik, Tuna, Olukman, Narli Ozdemir, Yildirim Sozmen, Sen, Akcicek, Duman: "The effects of vitamin d on gentamicin-induced acute kidney injury in experimental rat model." in: International journal of endocrinology, Vol. 2013, pp. 313528, (2013) (PubMed).

Details zu Lipocalin 2

Target: Lipocalin 2 (LCN2)

Andere Bezeichnung: LCN2 (LCN2 Produkte)

Synonyme: LCN2 ELISA Kit, 24p3 ELISA Kit, MSFI ELISA Kit, NGAL ELISA Kit, AW212229 ELISA Kit, Sip24 ELISA Kit, lipocalin 2 ELISA Kit, LCN2 ELISA Kit, Lcn2 ELISA Kit

Hintergrund:

Protein Function: Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5- DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth (By similarity). .

Background: Lipocalin-2(LCN2), also known as NGAL, is a protein associated with neutrophil gelatinase.1 The LCN2 gene is located at 9q34 and contains 7 exons.2 The 25-kD LCN2 protein is believed to bind small lipophilic substances such as bacteria-derived lipopolysaccharide(LPS) and formylpeptides and may function as a modulator of inflammation. NGAL tightly binds bacterial catecholate-type ferric siderophores through a cyclically permuted, hybrid electrostatic/cation-pi interaction and is a potent bacteriostatic agent in iron-limiting conditions.3 The primary LCN2 transcript is 3,696 nucleotides long, and the processed transcript is 809 nucleotides long.4 LCN2 expression in adult bone marrow, uterus, prostate, salivary gland, stomach, appendix, colon, trachea, and lung, and in fetal spleen and lung. The standard product used in this kit is recombinant rat NGAL, consisting of 177 amino acids with the molecular mass of 21 kDa.

Synonyms: Neutrophil gelatinase-associated lipocalin,NGAL,Alpha-2-microglobulin-related protein,Alpha-2U globulin-related protein,Lipocalin-2,Siderocalin LCN2,p25,Lcn2,

Full Gene Name: Neutrophil gelatinase-associated lipocalin

Cellular Localisation: Secreted . Upon binding to the SLC22A17 (24p3R) receptor, it is internalized..

Gen-ID: 170496

UniProt: P30152

Pathways: Cellular Response to Molecule of Bacterial Origin, Transition Metal Ion Homeostasis