IgY ELISA Kit
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- Target Alle IgY Produkte
- IgY
- Reaktivität
- Huhn
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Untere Nachweisgrenze
- 80 ng/mL
- Applikation
- ELISA
- Verwendungszweck
- The IgG(chicken)-ELISA is designed for the quantitative determination of chicken IgG in complex samples (serum or other biological samples).
- Proben
- Serum
- Analytische Methode
- Quantitative
- Produktmerkmale
- The determination of chicken IgG is carried out as direct sandwich ELISA.
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- Plattentyp
- Pre-coated
- Protokoll
- An antibody specific for chicken IgG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IgG present is bound. After washing away any unbound substances, an enzyme-linked antibody is added. Following a wash, a substrate solution is added to the wells and color develops in proportion to the amount of antibody conjugate. The absorption at 450 nm is proportional to the IgG concentration.
- Testdurchführung
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Incubation of samples/standards: 100 µL/well 1 hr with shaking or 2 hrs without shaking
Wash Incubation of detection antibody: 100 µL/well 1 hr with shaking or 2 hrs without shaking
Wash Substrate incubation: 100 µL/well 10 min, room temperature
Add stop solution: 100 µL/well
Read at 450 nm - Testpräzision
- Intraassay precision (CV):(n=10)at 50.8 ng/ml: 3.0%at 122.6 ng/ml: 6.6%
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Lagerung
- 4 °C
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- Target Alle IgY Produkte
- IgY
- Andere Bezeichnung
- IgG/IgY (IgY Produkte)
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