IgG ELISA Kit
-
- Target Alle IgG ELISA Kits anzeigen
- IgG
-
Reaktivität
- Ratte
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Untere Nachweisgrenze
- 156 ng/mL
- Applikation
- ELISA
- Verwendungszweck
- The IgG(rat)-ELISA is designed for the quantitative determination of rat IgG in complex samples (serum or other biological samples).
- Proben
- Serum
- Analytische Methode
- Quantitative
- Produktmerkmale
- The determination of rat IgG is carried out as direct sandwich ELISA.
- Featured
- Zu unserem meistverkauften IgG ELISA Kit
- Top Product
- Discover our top product IgG ELISA Kit
-
-
- Plattentyp
- Pre-coated
- Protokoll
- An antibody specific for rat IgG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IgG present is bound. After washing away any unbound substances, an enzyme-linked antibody is added. Following a wash, a substrate solution is added to the wells and color develops in proportion to the amount of antibody conjugate. The absorption at 450 nm is proportional to the IgG concentration.
- Testdurchführung
-
Incubation of samples/standards: 100 µL/well 1 hr with shaking or 2 hrs without shaking
Wash Incubation of detection antibody: 100 µL/well 1 hr with shaking or 2 hrs without shaking
Wash Substrate incubation: 100 µL/well 10 min, room temperature
Add stop solution: 100 µL/well
Read at 450 nm - Testpräzision
- Intraassay precision (CV): (n=10) at 73 ng/ml: 5.8% at 274 ng/ml: 8.9%
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Lagerung
- 4 °C
-
- Target Alle IgG ELISA Kits anzeigen
- IgG
- Abstract
- IgG Produkte
- Substanzklasse
- Antibody
-