FTH1 ELISA Kit

Produktnummer ABIN455102

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Maus FTH1.

Kurzübersicht für FTH1 ELISA Kit (ABIN455102)

Target: FTH1 (Ferritin, Heavy Polypeptide 1 (FTH1))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Proben: Urine, Serum, Plasma

Produktdetails FTH1 ELISA Kit

Verwendungszweck: This immunoassay kit allows for the specific measurement of Mouse Ferritin concentrations in urine ,serum and plasma.

Analytische Methode: Quantitative

Spezifität: This assay recognizes natural Mouse Ferritin.

Kreuzreaktivität (Details): No significant cross-reactivity or interference was observed.

Produktmerkmale: Mus musculus,Mouse,Ferritin heavy chain,Ferritin H subunit,Fth1,Fth,1.16.3.1

Anwendungsinformationen

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll: This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Ferritin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Ferritin present is bound by the immobilized antibody. An enzyme-linked polyclonal antibody specific for Ferritin is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Ferritin bound in the initial step. The color development is stopped and the intensity of the color is measured.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.

Details zu FTH1

Target: FTH1 (Ferritin, Heavy Polypeptide 1 (FTH1))

Andere Bezeichnung: Fth1

Hintergrund: Ferritin is an iron-protein complex formed from an intracellular acceptor called Apoferritin. Apoferritin is a large molecular weight 450,000 protein produced by the liver. Iron as Fe (HO)3 linked to apoferritin is then stored in the cytoplasm of the reticuloendothelial system, liver, spleen and bone marrow. Ferritin is the body's iron storage protein functioning primarily as a site for iron storage from which iron may be mobilized in response to such stimuli a dietary change, blood loss or pregnancy. The direct quantitation of serum ferritin offers the physician a convenient and accurate measure of total body iron stores, by means of diagnosing iron-deficiency and anemia due to such causes as inflammation and hepatic or renal disease. In addition, serum ferritin concentration may be useful in detecting iron overload, which may allow the detection of idiopathic hemachromatosis in the precirrhotic storage.

Pathways: Transition Metal Ion Homeostasis