ALDH1A1 ELISA Kit

Produktnummer ABIN4881794

Produktdetails ALDH1A1 ELISA Kit

Target: ALDH1A1 (Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Custom Human ALDH1A1 ELISA Kit.

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu ALDH1A1

Target: ALDH1A1 (Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1))

Andere Bezeichnung: ALDH1A1 (ALDH1A1 Produkte)

Synonyme: ALDC ELISA Kit, ALDH-E1 ELISA Kit, ALDH1 ELISA Kit, ALDH11 ELISA Kit, PUMB1 ELISA Kit, RALDH1 ELISA Kit, AL1A1 ELISA Kit, aldc ELISA Kit, aldh-e1 ELISA Kit, aldh1 ELISA Kit, aldh11 ELISA Kit, pumb1 ELISA Kit, raldh1 ELISA Kit, GGADHR ELISA Kit, ALDH1A1 ELISA Kit, ALDDH ELISA Kit, Ahd2 ELISA Kit, Aldh1 ELISA Kit, Aldh2 ELISA Kit, Ahd-2 ELISA Kit, Aldh1a2 ELISA Kit, E1 ELISA Kit, Raldh1 ELISA Kit, Aldh1a1 ELISA Kit, ALHDII ELISA Kit, RALDH 1 ELISA Kit, RalDH1 ELISA Kit, aldehyde dehydrogenase 1 family member A1 ELISA Kit, aldehyde dehydrogenase 1 family, member A1 ELISA Kit, aldehyde dehydrogenase 1 family member A1 L homeolog ELISA Kit, aldehyde dehydrogenase family 1, subfamily A1 ELISA Kit, aldehyde dehydrogenase ELISA Kit, retinal dehydrogenase 1 ELISA Kit, ALDH1A1 ELISA Kit, aldh1a1 ELISA Kit, Aldh1a1 ELISA Kit, aldh1a1.L ELISA Kit, aldH1 ELISA Kit, LOC100732581 ELISA Kit, LOC101822890 ELISA Kit

Gen-ID: 216

UniProt: P00352

Pathways: Dopaminergic Neurogenesis