ARNT ELISA Kit

Produktnummer ABIN4881888

Produktdetails ARNT ELISA Kit

Target: ARNT (Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Custom Human ARNT/HIF-1 beta ELISA Kit.

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes .

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu ARNT

Target: ARNT (Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT))

Andere Bezeichnung: ARNT (ARNT Produkte)

Synonyme: HIF-1-beta ELISA Kit, HIF-1beta ELISA Kit, HIF1-beta ELISA Kit, HIF1B ELISA Kit, HIF1BETA ELISA Kit, TANGO ELISA Kit, bHLHe2 ELISA Kit, D3Ertd557e ELISA Kit, Drnt ELISA Kit, ESTM42 ELISA Kit, Hif1b ELISA Kit, W08714 ELISA Kit, mKIAA4051 ELISA Kit, Arnt1 ELISA Kit, ARNT ELISA Kit, zgc:136664 ELISA Kit, arnt1 ELISA Kit, hif1b ELISA Kit, tango ELISA Kit, xArnt ELISA Kit, hif1beta ELISA Kit, hif-1beta ELISA Kit, arnt.2 ELISA Kit, MGC68805 ELISA Kit, aryl hydrocarbon receptor nuclear translocator ELISA Kit, aryl hydrocarbon receptor nuclear translocator S homeolog ELISA Kit, ARNT ELISA Kit, Arnt ELISA Kit, arnt ELISA Kit, arnt.S ELISA Kit

Gen-ID: 405

UniProt: P27540

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, Warburg Effekt